TRFIA检测甲状腺功能出现假阳性的因素分析.docVIP

精品论文 参考文献 TRFIA检测甲状腺功能出现假阳性的因素分析 吴广忠 （江苏省南通市通州区第八人民医院检验科 226361） 【摘要】目的 探讨时间分辨免疫荧光分析（TRFIA）检测甲状腺功能过程中存在假阳性的因素分析。方法 回顾性统计分析了56例用TRFIA方法检测的甲状腺功能亢进血清标本，再采用化学发光免疫分析(CLIA)方法定量检测甲状腺功能亢进血清标本，进行比对试验，确定TRFIA方法的假阳性率，并分析影响TRFIA方法出现假阳性的影响因素及其相应处理方法。结果 TRFIA方法检测的56例甲状腺功能亢进血清标本,分别送上级医院用贝克曼、雅培、罗氏、拜耳四个生产厂家的检测仪器进行复检后，52例仍为甲状腺功能亢进，6例甲状腺功能正常，表明TRFIA法有89.6％的真阳性率，有10.4％的假阳性率,TRIFA与化学发光法两者符合率为89.6%。结论 TRFIA出现假阳性的影响因素除了方法学、试剂盒本身之外，还有标本处理、操作不当等多种因素。以上多种因素会导致TRFIA法检测甲状腺功能出现假阳性，对可疑的临床症状不符合甲状腺功能亢进的血清标本,除了严格操作、做好质控外，一定要认真复测，最好采用不同厂家化学发光仪器进行复查,以排除方法学差异出现的假阳性结果。 【关键词】时间分辨免疫荧光分析(TRFIA) 甲状腺功能亢进 化学发光法(CLIA) 假阳性 【中图分类号】R446 【文献标识码】A 【文章编号】2095-1752（2013）24-0369-02 【Abstract】 Objective To investigate the time-resolved fluoroimmunoassay (TRFIA) analysis of false positive factors existing in the process of detection of thyroid function. Methods: the retrospective analysis of 56 cases by TRFIA method for detection of thyroid function hyperfunction serum samples, followed by chemiluminescent immunoassay (CLIA) method for quantitative detection of thyroid function hyperfunction serum samples, were compared to the test, to determine the false positive rate of TRFIA method, TRFIA method and to analyze the impact of the factors which influence the false positive and the corresponding treatment method. Results: the TRFIA method for the detection of 56 cases of hyperthyroidism serum specimens, recheck testing instruments were sent to the hospital, with Backman Roche, Abbott, Bayer four manufacturers, 52 patients still hyperthyroidism, 6 cases of normal thyroid function, TRFIA method shows that the true positive rate 89.6%, false positive rate 10.4%, TRIFA and chemiluminescence method, the coincidence rate was 89.6%. conclusion: TRFIA the factors which influence the false positive in addition to methods, kits, and sample processing, improper operation and other factors. These factors will lead to TRFIA method for detection of thyroid function of f