丹参素对大鼠局灶性脑缺血再灌注损伤保护作用.docVIP

丹参素对大鼠局灶性脑缺血再灌注损伤保护作用摘要：目的研究丹参素对大鼠局灶性脑缺血再灌注损伤的保护作用，并初步探讨其作用机制。方法用线栓法制备大鼠局灶性脑缺血再灌注损伤模型，通过神经功能学评分、苏木素和伊红（HE）染色及流式细胞术检测丹参素对大鼠脑缺血再灌注损伤后行为学、缺血侧脑组织病理形态学及神经细胞凋亡的影响。结果丹参素可明显改善脑缺血大鼠神经功能缺损体征，减轻脑组织病理形态学损伤，降低神经细胞凋亡百分率。结论丹参素对大鼠局灶性脑缺血再灌注损伤具有保护作用，其作用机制可能与抑制神经细胞凋亡有关。 关键词：丹参素；脑缺血；再灌注损伤；细胞凋亡 中图分类号：R743.31文献标识码：A文章编号：1672-979X(2009)01-0024-03 Protective Effect of Danshensu on Focal Cerebral Ischemia-reperfusion Injury in Rats YANG Ying1,XUE Xia2 , LIU Zhao-ping2* (1. Experimental Teaching Center, Shandong University of Traditional Chinese Medicine, Jinan 250014, China; 2. Center for New Drugs Evaluation, Shandong University, Jinan 250012, China) Abstract：Objective To investigate the protective effect of danshensu on focal cerebral ischemia-reperfusion injury in rats and initially approach it?s mechanism. Methods The Wistar rat model of focal cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion (MCAO). Behavior examination was used to evaluate the damage to central nervous system. Hematoxylin and eosin (HE) stain was used to observe the pathological change of ischemic brain tissue. Flow cytometry (FCM) was employed to determine neural cell apoptosis. Results Danshensu could remarkably ameliorate neurologic impairment in rats with cerebral ischemia, relieve pathological change of ischemic brain tissue and decrease the percentage of neural cell apoptosis. ConclusionDanshensu has a protective effect on focal cerebral ischemia-reperfusion injury in rats. The mechanism may be related to the inhibition of neural cell apoptosis. Key words：danshensu; cerebral ischemia; reperfusion injury; apoptosis 中药丹参是唇形科多年生草本植物丹参的干燥根，具有活血凉血、祛瘀止痛、安神除烦之功效。丹参素是从中药丹参提取的主要有效成分之一，以丹参素为主要成分的丹参注射液、复方丹参滴丸已广泛用于临床治疗心脑血管病、肝肾损伤、炎症、休克等疾病。文献报道，丹参素对大鼠心肌缺血再灌注损伤有保护作用[1]，但关于丹参素对脑缺血再灌注损伤（cerebral ischemia-reperfusion injury，CIRI）保护作用的研究较少。本研究用大脑中动脉栓线法（middle cerebral artery occlusion，MCAO）制备局灶性脑缺血再灌注损伤模型，通过神经功能学评分、苏木素和伊红（HE）染色及流式细胞术观察丹参素对局灶性脑缺血再灌注损伤大鼠行为学、缺血侧脑组织病理形态学及神经细胞凋