H4亚型禽流感病毒套式 RTPCR 检测方法的建立.pdfVIP

Progress in V eterinary M edicine H R T P C R H A IV H A IV HA H A IV RT PCR H A IV H A IV f g L RT PCR H A IV H A IV RT PCR S Q A Avian Inf luenza A I A Avian Inf luenza Vi B A H J H H PCR J H N A IV RT PC R J J Simultaneous Detection of HN Subtypes and M Gene of Avian Influenza Virus by Multiplex PCR LIU T ing ting XIE Zhix un SON G De ui L U O Sisi XIE Lii g j LI M eng XIE Zhi ing DEN G Xianw eng q G uang x i N o r m al Univ e rs ity Co lle ge of l if e Sc ience G uil in G uang x i China G uang x i Vete r inar y R ese arch I ns t it ute G uang x i K ey L abo r ato r y of A nim al V acc ines and N ew Techno lo g y N anning G uang x i China Abstract In order to establish a method to detect avian inf luenza virus A IV and separating H N sub ty pes three pair of specific primer w ere desig ned and selected according to the conserved sequences of A IV matix M enethe hemagglutinin HA enes of H subty pe A IV and the Neuraminidase N A enes of g g g N subty pe A IV the reaction conditions w ere optimized and established the multiplex RT PCR T o f ur ther evaluate the reliability the specificity and sensitivity of assay w ere evaluated and clinical samples