大鼠急性脑缺血及缺血再灌注中erk5的表达情况.docVIP

精品论文 参考文献 大鼠急性脑缺血及缺血再灌注中ERK5的表达情况 谭中旭1 宋玉强（通讯作者）2 吕敬雷3 于力群1 时圣桂1 　　(1青岛大学医学院 山东 青岛 266021) 　　(2青岛大学医学院附属医院神经内科 山东 青岛 266003) 　　(3青岛大学医学院附属医院神经功能检查科 山东 青岛 266003) 　　【摘要】 目的：观察大鼠脑皮质中细胞外信号调节激酶5(ERK5)在缺血及缺血再灌的不同表达，探讨其在脑缺血再灌注损伤中的意义。方法：采用线栓法建立大脑中动脉闭塞( MCAO) 模型，所有大鼠随机分为假手术组、缺血梗死组和缺血再灌组。对各组大鼠行神经功能评分，TTC染色测定脑梗死体积，免疫组织化学法测定ERK5表达。结果：缺血再灌注组大鼠的神经功能缺损和脑梗死体积均比缺血梗死组大鼠严重。ERK5在缺血梗死组和缺血再灌组都表达，后者明显多于前者。结论：ERK5可能参与脑缺血再灌注损伤过程并起着重要作用。 　　【关键词】 脑缺血；缺血再灌注损伤;细胞外信号调节激酶5(ERK5);表达 　　【中图分类号】R742 【文献标识码】A 【文章编号】2095-1752（2016）10-0172-02 　　Expression of ERK5 in rats cortex afer cerebral ischemia and reperfusion TAN Zhongxu1,SONG Yuqiang2,LV Jinglei3,YU Liqun1,SHI Shenggui11Qingdao University Medical College,Qingdao 266021,China.2Department of Neurology,the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003,China .3 The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China 　　【Abstract】Objective To observe the different expression of ERK5 in rats cortex in response to cerebral ischemia and ischemia/reperfusion, and investigate the significance in ischemia/reperfusion injury.Methods Blocking middle cerebral artery with intraluminal thread was used to build cerebral middle artery infarction model ( MCAO).All rats were randomly divided into sham operation group,ischemia groupand ischemia/reperfusion (I/R)group.The Longa score was used to evaluate revived rats and TTC staining was used to determine the infarct volumes.we investigated the activation and expression of ERK5 by immunohistochemistry.Results 24 hours after MCAO, neurological function scores were significant increased in I/R group compared with ISCH group. the infarct volume was in I / R group was larger.The activation of ERK5 was rapidly induced by ischemia, and I/R group were significantly higher than Ischemia group.Conclusion We hypothesize that ERK5 might participate in the pathogenesis of isehemic/reperfusion and play an importan