大鼠急性胰腺炎早期血清mir－２１的变化及意义的研究.docVIP

精品论文 参考文献 大鼠急性胰腺炎早期血清miR－２１的变化及意义的研究 罗　波　蒋益泽　魏　东 　　成都中医药大学附属医院　四川　成都　６１００７２ 作者简介:罗波,男,(１９８０－),硕士研究生,主治医师,主要研究方向:肝胆外科疾病研究. 　　【摘要】　目的　探索miR－２１在大鼠急性胰腺炎早期的变化及其临床意义.方法３６只SD大鼠分为３组,A 组(出血坏死性胰腺炎组)、B组(水肿性胰腺炎组)和C组(对照组),用１％和５％的牛黄胆酸钠沿胰胆管逆行注射,构建大鼠水肿性和出血坏死性胰腺炎模型.检测三组大鼠血清中淀粉酶、脂肪酶、IL－１ 和IL－６的变化,并做胰腺病理评分.用RT－PCR法检测三组大鼠血浆中的miR－２１的含量,用免疫组织化学法检测胰腺中的PTEN 的表达.结果A 组和B 组的血清淀粉酶、脂肪酶、IL－１、IL－６水平高于C组,差异有统计学意义(P＜０．０５);胰腺病理评分A 组高于B组和C组,差异有统计学意义(P＜０．０５);miR－２１在A 组的表达低于B组和C组,差异有统计学意义(P＜０．０５);PTEN 表达在A 组高于B组和C组,差异有统计学意义(P＜０．０５).结论miR－２１可能成为急性出血坏死性胰腺炎早期病情预测的指标,PTEN 在急性胰腺炎炎症的调控方面可能发挥了重要作用. 【关键词】　急性胰腺炎;胰腺炎模型;miR－２１;PTEN 蛋白StudyonChangesandFunctionofmiR－２１inSerumofRatsinEarlyStageofAcutePancreatitis　LuoBo,JiangYize,WeiDong 　(TheAffiliated HospitalofChengduUniversityofTraditionalChineseMedicine,Chengdu６１００７２,Sichuan,China)【Abstract】Objective:ToexplorethechangesofmiR－２１inratswithacutepancreatitisattheearlystageanditsclinicalsignificance．Methods:３６SDratsweredividedrandomizedlyintothreegroups．GroupAwerehemorrhagenecrotizingpancreatitisgroup．GroupBwereedematouspancreatitisgroup．GroupCwerethecontrolgroup．Theedematousandnecrotizingpancreatitismodelinratwasmadewith１％and５％sodiumtaurocholatealongtheretrogradepancreaticductinＧjection．Thecontrolgroupwasblankcontrol．Thelevelofamylase,lipase,IL－１andIL－６weretestedandthepancreaspathologicalscorewasassessed．ThelevelofmiR－２１wastestedbyRT－PCRandthePTENproteininpancreaswastestedbyimmunohistochemistry．Results:Theleavelofamylase,lipase,IL－１andIL－６ingroupAandBwerehigherthangroupC,thedifferencewasstatisticallysignificant(P＜０．０５)．ThepathologicalscoreofpancreasinAgroupwashigherthanthatinBgroupandCgroup,thedifferencewasstatisticallysignificant(P＜０．０５)．TheleavelofmiR－２１ingroupAwaslowerthangroupBandgroupC(P＜０．０５)．TheleavelofPTENproteiningroupAwashigherthangroupBandgroupC(P＜０．０５)．Conclusion:MiR－２１maybeaindicatorofacutehemorrhageneＧcrotizingpancreatitisintheearlystage．PTENproteinmayplayanimportantroleintheregulationofinflammationinacutepancreatitis．【Keywords】Acutepancreatitis;Pancr