RhoC及MMP-9在原发性肝细胞癌中表达水平及术后复发关系探究.docVIP

RhoC及MMP-9在原发性肝细胞癌中表达水平及术后复发关系探究 　 作者：潘乐玉　滕木俭　时昌文　王明海 【摘要】 目的： 探讨RhoC及基质金属蛋白酶-９（metrix metalloproteinases, MMP-9）蛋白在原发性肝细胞肝癌（以下称肝癌）发生、发展、转移及术后复发过程中的作用。方法：采用免疫组织化学染色检测43例肝癌患者术后癌灶和癌周标本及25例肝硬化、23例正常肝脏标本中两种蛋白的表达情况，分析两种蛋白的表达与肝癌病理特点的关系及两者表达的相关性，采用COX比例风险模型进行肝癌术后复发风险多因素分析。结果：RhoC在肝癌组与癌旁组、肝硬化组、正常肝组及癌旁组与正常肝组表达程度差异有统计学意义（Plt;0.05）；MMP-9在肝癌组与癌旁组、肝硬化组、正常肝组表达程度差异有统计学意义（Plt;0.05）。RhoC与MMP-9在肝癌癌灶中表达存在相关性，且为正相关（rs’=0.495， Plt;0.01）。RhoC阴性组的中位生存时间46个月，阳性组中位生存时间为20个月；MMP-9阴性组的中位生存时间49个月，阳性组的中位生存时间25个月； RhoC及MMP-9都为阴性，中位生存时间56个月；RhoC或MMP-9为阴性，中位生存时间39个月；RhoC及MMP-9都为阳性，中位生存时间19个月。COX比例风险分析模型分析认为：肿瘤侵及门静脉（包括微转移灶）、淋巴结转移、RhoC及MMP-9阳性表达分别为肿瘤术后复发的独立危险因素。结论：RhoC蛋白在肝癌的早期开始起作用，且RhoC蛋白及MMP-9蛋白的异常高表达提示肿瘤恶性程度高，术后复发率高，指导临床术后预防性化学治疗有一定指导意义。 【关键词】 癌，肝细胞·RhoC蛋白·基质金属蛋白酶类·复发 【ABSTRACT】 Objective: To explore the value of ras homologous C （RhoC) and matrix metalloproteinase-9 （MMP-9) protein in the occurrence, development, metastasis and recurrence of primary hepatocellular carcinoma （HCC). Methods: To investigate the expression of the two proteins in the specimans of 43 HCC, 43 paracancer, 25 cirrhosis and 23 normal liver tissues by immunohistochemistry, and analyse the relation between the expression and the pathologic features of HCC and the expression among the different tissues, including the correlation between the two proteins. COX proportion hazard model was used to estimate the recurrence risk factors. Results: The expression of RhoC protein in specimans of HCC was significantly higher than paracancer, cirrhosis and normal liver tissues, the difference between paracancer and normal liver tissues was also significant. The expression of MMP-9 protein in specimens of HHC was significantly higher than paracancer, cirrhosis and normal liver tissues. There was positive correlation between the expressions of the two proteins. （rs=0.495 Plt;0.01) .The median survival time in negative group of RhoC protein was 46.00 months and the positive one was 20.00 months. The median survival time in negative one o