杨树叶试管不定根高频率同步发生体系的研究.pdfVIP

安徽农业科学，Journal ofAnhuiAgn．Sci．2008，36(14)：5782—5785 责任编辑 姜丽 责任校对 马君叶 杨树叶试管不定根高频率同步发生体系的研究 马庆国 ，董宁光2，魏丽z，段承俐 ，裴东z* (1．云南农业大学，云南昆明650201；2．中国林业科学研究院林业研究所，北京 100091) 摘要 『目的]建立高频率和高同步性不定根发生体系。[方法】以长期继代培养的741杨无根苗或有根试管苗[Populus alba X(尸． davidian0+P siraonni)P．t0 ntosa]的叶、嫩茎、叶盘和叶柄等外植体为试材，在试管内分别诱导不定根发生。[结果]从不定根发根时 间、生根率和生根同步性等方面看，所有生根试管苗上的叶明显优于嫩茎、继代苗上的叶、叶盘和叶柄，而试管生根初期苗上的叶又 好于生根后期苗上的叶。进一步优化试管生根初期苗上的叶的不定根诱导条件，在1，2 MS+0．5 mg／LIBA生根培养基上，培养6 d可见 根原基突起，6-8 d为不定根高发期，8 d后生根率可达100％，单叶平均生根数为3．7条。不定根发生进程的形态学观察发现，不定根 发生部位为叶柄基部及向上至3 mm处，不定根起始分化时间集中为诱导后2 d，不定根发生起源于维管形成层，特别是维管射线正 对的形成层。『结论]杨树是木本植物的模式植物，建立高频率和高同步性不定根发生体系，可为深入研究木本植物不定根发生调控机 制提供良好的试验体系和技术平台。 关键词 杨树；不定根；IBA；叶 中图分类号 S722．3 文献标识码 A 文章编号 0517—6611(2008)14—05782—04 Study on High Efficiency Induction and High Synchronism System of Adventitious Root of Po·plar Leaves in vitro MA Qing~uo et al Yunnan Agrl cultural University，Kunming,Yunnan 650201) Abstract [Objective]The research aimed to establish high efficiency induction and high synchronism system of adventitious root in Poplar． [Method]Taking leaves，stems，leaf discs and petioles of rooted plantlets and subculture in vih-o of poplar 741[Populus alba X(P davidiana+P simonni)P．tomentosa]as experiment materials，the induction situation of adventitious root was studied．『Result]Leayes from rooted plantlets were obviously better than that of other explants like stem cuttings，leaf discs and petioles in aspects like time of rooting，rooting rate and synchronism． When 0．5 mgL IBA was applied in leaves from early days rooting plantlets，the adventitious root started at the 6th day…and the rooting rate was up to 100％at the 8th day．and the average number of root was 3．7．Adventitious roots differentiated after the induction time was 2 days and the rooting site was 0．3 mm to the bottom of the petioles．Adventitious root came from fascicular cambium especia~y which was opposite to the vascular ray：[Conclusion]The