高效液相色谱法测定家兔肌肉及组织中土霉素、四环素的残留量.pdfVIP

虿北荩 业学报 2008，17(3)：58—60 Acta Agriculturae Boreali-occidentalis Sinica 高效液相色谱法测定家兔肌肉及组织中 土霉素、四环素的残留量 张丽云，白雪梅 (河北北方学院，张家口 075000) 摘 要：用高效液相色谱(High performance liquid chromatography，HPLC)法测定家兔腿肌、心、肝、肾等组 织中土霉素、四环素残留量。色谱柱为Hypersil BDS，以乙睛一3 冰乙酸水溶液(30：70)为流动相，检测波 长为350 D．m，柱温3O℃。结果表明：土霉素、四环素标准品浓度在 8．2～262．5／~g／mL范围内线性关系良好， 土霉素线性方程为y一 19392．82X一22．90，r一0．99948；四环素线性方程为y一14089．84X一33．34，r一 0．99888。家兔肝脏的土霉素加样回收率平均为89．1％，RSD=2．O3 ；四环素加样回收率平均为 91．1 ， RSD=2．22 。说明HPI C法准确可靠，为监控兔产品土霉素、四环素残留提供了安全、方便的检测手段。 关键词：高效液相色谱法；家免；土霉素；四环素 中图分类号：R155．55；0657．72；R978．14 文献标识码：A 文章编号：1004—1389(2008)03—0058—03 Simultaneous Determination of oxytetracycline，Tetracycline in Meat and Tissue of Rabbit by High Performance Liquid Chromatography ZHANG Li— yun and BAI Xue——mei (Hebei Nor*h University，Zhangjiakou Hebei 075000，China) Abstract：Determination the contenl of()xytetracycline，tetracycline in leg meat，heart，liver and kid— ney of rabbit by high performance liquid chromatography．oxytetracycline and tetracycline was sepa— rated on a Hypersil BDS column using acelonitrile一3 ice—acetic acid(30：70)as mobile phase and detective wavelength at 350 nm．The linear correlation of tested oxytetracycline and tetracycline con— centration within the range of 8．2--262．5／~g／mL was idea1．Linear equation of oxytetracycline was Y 一 19392．82X一22．90，r一 0．99948：Linear equation of tetracycline was y一 14089．84X一 33．34，r一 0．99888．The average~ecovery rate of oxytetracycline and tetracycline in breast meat was 89．1 (RSD 一 2．03 )and 91．1 (RSD=2．22 )respectively．HPLC method is accurate，safety and convenient of the supervisory control for green non-pollution rabbit． Key words：High performance liquid chromatography；Rabbit