褪黑素对睡眠的调适作用及与脑内氨基酸递质的关系.pdfVIP

·生物化学· 褪黑素对睡眠的调节作用及与脑内氨基酸递质的关系 ( ) 王 　芳 　李经才 　徐 　峰 　何 　瑜 　唐 　超 沈阳 110016 沈阳药科大学时间生物学室 摘要 　目的 :研究褪黑素促眠、昼夜节律作用及其与脑内氨基酸递质的关系。方法 : 以翻正反射为指标研究褪黑素 γ ( ) 对小鼠睡眠及其昼夜节律的影响。采用纸电泳双波长扫描法 ,测定褪黑素对小鼠不同脑区中 氨基丁酸 GABA ( ) 和谷氨酸 Glu 含量的影响。结果 :褪黑素能明显促进小鼠睡眠 ,其药效强度依赖于给药的时辰。褪黑素可使小鼠 γ γ 下丘脑内 氨基丁酸含量明显升高。但对谷氨酸水平无明显影响。结论 :褪黑素促眠作用可能与小鼠下丘脑中 氨基丁酸含量升高有关。 γ 关键词 　褪黑素 ;睡眠 ;昼夜节律 ; 氨基丁酸 ;谷氨酸 The effects of melatonin on sleep and its relationship with amino acid neurotransmitters in brain in mice Wang Fang ( Wang F ) ,Li Jingcai (Li J C ) , Xu Feng ( Xu F ) , et al ( Shenyang Pharmaceutical University Chronobiology L aboratory , Shenyang 110016) ( ) ABSTRACT 　OBJECTIVE :To study the effects of melatonin MT on sleep and amino acid neurotransmitters in brain. METHODS :To determine the sleep time and circadian sleep rhythm by observing the loss of the righting reflex method in mice. The concentration of amino acid neurotransmitters( GABA ,Glu) in brain was determined by the paper electrophoretic and dual wavelength thin layer scanning method. RESUL TS :Melatonin could significantly prolong sleep time induced by sodium pentobarbital and markedly elevate the level of GABA but not of Glu in hypothalamus.CO NCL USIO N :It supposed that the effect of melatonin on sleep might be related to elevated level of GABA which is one of its mechanisms in mice. KEY WO RDS 　melatonin ,sleep ,circadian rhythm ,GABA ,glutamic acid ( ) ( ) ( 　　褪黑素 melatonin ,MT