维生素A缺乏易致老年黄斑变性实验研究.docVIP

维生素A缺乏易致老年黄斑变性实验研究 　　[摘要] 目的：通过对老龄小鼠眼局部组织中自由基的测定，探索维生素A缺乏对老年黄斑变性的诱导机制，为临床预防和治疗老年黄斑变性提供一定理论依据。方法：将昆明小鼠随机分为对照组、维生素A缺乏组和维生素A添加组，各组分别用相应饲料喂养50 d，取眼球组织测定组织中自由基的含量。结果：维生素A添加组小鼠的SOD、GSH-PX、CAT活性明显高于维生素A缺乏组和对照组（P＜0.05），同时MDA含量低于维生素A缺乏组和对照组，但无统计学意义。结论：老年黄斑变性的发生与维生素A缺乏有一定的关系。 　　[关键词] 维生素A;老年黄斑变性;实验研究 　　[中图分类号] R774.5[文献标识码]A [文章编号]1673-7210（2008）08（c）-032-02 　　 　　The experimental study of the aged macular degeneration for Vitamin A deficiency 　　LIANG Shan, LIANG Yun, SHEN Guo-yan 　　(The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang550002，China) 　　[Abstract] Objective：To explore induced mechanism age macular degeneration for Vitamin A deficiency through determining free radicals of the aged mice ocular tissues, and to provide a theoretical basis for the prevention and treatment of the aged macular degeneration. Methods：The mice were randomly divided into the control group，the lacked of vitamin A group and the added vitamin A group, to feed each group with corresponding animal feeds for 50 days，and to determine the content of free radicals in organization of the eyeball. Results： The SOD, GSH-PX and CAT of the added vitamin A group were obviously higher than the lacked of vitamin A group and the control group， and the content of MDA was lower than the lacked of vitamin A group and the control group（P＜0.05）.However,there was no significant statistical significance. Conclusion： The Aged macular degeneration have a certain relationship to vitamin A deficiency. 　　[Key words] Vitamin A; The aged macular degeneration; Experimental study 　　 　　老年黄斑变性（AMD）是以视力下降，视物变形和黄斑变性为主要临床特征的眼科疾病。目前AMD的发病率有逐年增长趋势，现代医学对AMD病因病机尚未完全明确，但普遍认为与年龄、遗传、营养、光照因素有关。维生素A是一种不饱和一元醇，分子中的双烯共轭键是单线态???、羟自由基、脂质过氧自由基以及其他自由基有效的猝灭剂和捕捉剂，从而保护机体免受过氧化物的损伤。有研究已证实维生素A在体外试验中是一种有效的抗氧化和清除自由基物质，并参与视紫红质的合成，增强视网膜感光力[1-2]。本文拟通过对老龄小鼠眼局部组织中自由基的测定，探索维生素A缺乏对老年黄斑变性的诱导机制，为临床预防和治疗老年黄斑变性提供一定理论依据。 　　1材料与方法 　　1.1动物分组与饲养 　　采用贵阳医学院动物中心提供成年昆明种小鼠60只，体重30～50 g，适应性喂养3 d后随机分成3组：对照组和实验组，实验组又随机分为维生素