维生素E和维生素C联合应用对慢性镉中毒小鼠黑质神经元保护作用.docVIP

维生素E和维生素C联合应用对慢性镉中毒小鼠黑质神经元保护作用 　　【摘要】 目的 探讨维生素E(Vit E)和维生素C(Vit C)联合用药对慢性镉中毒小鼠黑质神经元的保护作用。方法 小鼠染镉同时给予大量Vit E和Vit C，通过光镜观察小鼠黑质神经元的细微结构，并用细胞形态计量方法对黑质区酪氨酸羟化酶(TH)阳性反应神经元进行定量分析。结果 Vit E和Vit C加镉组小鼠中脑黑质区结构接近正常对照组，与镉中毒组比较有明显不同，黑质神经元胞核体密度、数密度等形态学参数与镉中毒组比较差异有统计学意义。结论 抗氧化剂Vit E和Vit C对慢性镉中毒组小鼠黑质神经元有显著的保护作用。 　　【关键词】 镉；维生素E；维生素C；黑质；细微结构；小鼠 　　 　　Protective effects of co-administration of Vitamin E and Vitamin C on the neurons of substantia nigra in chronic cadmium poisoning miceYU Yan?*，GE Guo，XU Ting-liang,et al．Department of Anatomy，Guiyang Medical College,Guiyang 550004，China 　　【Abstract】 Objective To study the protective effects of co-administration of Vitamin E and Vitamin C on the neurons of substantia nigra in chronic cadmium poisoning mice．Methods Mice were givenCdCl?2 subcutaneously and simultaneously were administered Vitamin E perorally everyday,Vitamin C perorally everyday．It has been observed that the microstructure of the substantia nigra under light microscopy，the TH-positive reaction neurons in the substantia nigra were quantitatively analyzed with cell morphometric technique．Results The co-administration of Vitamin E and Vitamin C with cadmium group was similar to the contral group，but it had much apparent changes as compared with the Cd-exposed group, and the important morphological parameters such as the volume density，the numerical density of the nudei had statistical significant compared with the Cd-exposed group(P＜0．001)．Conclusion The antioxidants of Vitamin E and Vitamin C could efficiently protect the neurons of substantia nigra in chronic cadmium poisoning mice from such damage． 　　【Key words】 Cadmium；Vitamin E； Vitamin C； Substantia nigra； Microstructure； Mouse 　　 　　镉(cadmium，Cd)是一种有毒重金属，镉污染对人体和环境的影响已日益受到重视。近年来发现镉对神经系统也有明显的毒性作用[1-2]，即使含量低时也有不同程度的损害[3]。我们的前期工作也表明，慢性镉中毒可致小鼠中脑黑质受损，并提示镉可能是诱发帕金森病的病因之一[4]。Vit E和Vit C是有效的抗氧化剂，国内外许多资料已表明Vit E和Vit C可保护神经组织免受Cd引起的自由基增多和氧化反应异常所造成的损害[5-7]，但是有关形态学方面的研究甚少。本研究通过对正常组、Cd中毒组、Vit E+Vit C组小鼠中脑黑质神经元进行光镜及细胞形态计量分析，探讨Vit