血液pea检测鉴别pa感染与定植的实验分析-experimental analysis of blood pea detection to identify pa infection and colonization.docxVIP

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血液pea检测鉴别pa感染与定植的实验分析-experimental analysis of blood pea detection to identify pa infection and colonization.docx

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血液pea检测鉴别pa感染与定植的实验分析-experimental analysis of blood pea detection to identify pa infection and colonization

expression serum cell factors TNF alpha (tumor necrosis factor alpha), IL-6 (interleukin (IL) 6) the differences of clinical value. Methods: 54 only clean type male SD rats by luzhou medical school center for animals. All shall be dexamethasone rats sodium 3 mg/kg, subcutaneous injection of 7 days. Randomly divided into infection model group (hereinafter referred to as the model group, and only from the trachea, into two McIntosh units of PA bacteria liquid 0.4 ml), oropharynx engraftment group (hereinafter referred to as the planting group, 18, only from the throat and nasal drops into the and model group the same concentration of bacteria liquid 0.2 ml), physiological saline control group (hereinafter referred to as the control group, and only from the trachea, into the physiological saline 0.4 ml). In the third, 7 after inoculation, 11 days were executed only 6 rats, take engraftment group throat swabs smear culture observation PA colonies, left the lung homogenate and diluted training count bacteria, under the right YeHang HE sliced and to observe the degree dyeing inflammation. Heart picks, with real-time fluorescence quantitative polymerase chain reaction(real-time fluorescent quantitative polymerase chain reaction,FQ-PCR) testing blood PEA relative amount expression, ELISA test (enzyme-linked immunosorbnent assay, ELISA) was serum TNF alpha, IFN-r, IL-6, IL-8 concentration.Results: 1, the bacteria cultures: planting group throat swabs were visible PA bacterial growth, among them with 3 and 7 days after inoculation is the maximum number of bacteria; 2, lung tissue culture: model group in three time points are seen large PA colonies, including 3 and 7 days number 1 colony in cfu/lung, 11 days 1 03 cfu/lung, engraftment group in different time points only a fewbacteria growth, and the control group did not see the lung tissue culture PA bacteria; 3, pathological observation: model group in the third, 7 days alveolar inflammation degree is heavy, see a large in