HPLC法分析不同产地生何首乌中的蒽醌类化合物.docVIP

精品论文 参考文献 HPLC法分析不同产地生何首乌中的蒽醌类化合物 潘文嘉(通讯作者) 林双 黎文敏 林坚涛（广东医学院 广东东莞 523808） 【摘要】目的：采用反相高效液相色谱法测定十个已知产地的生首乌水解前、后蒽醌类成分的含量，从而评价不同采集地生何首乌的质量。方法：HPLC法，以C18为色谱柱，甲醇-0.1%磷酸（85：15）为流动相，流速为1.0mlbull;min-1；检测波长254nm；结果：四川峨眉山样品中的蒽醌类成分含量最高，游离型和结合型大黄素含量分别为235.59mg/100g，18.17mg/100g，游离型大黄素甲醚为97.69 mg/100g；而重庆缙云山、贵州施秉、广西田阳样品中蒽醌类成分含量则较低。结论：各产地生何首乌中蒽醌类成分主要为大黄素，大黄素甲醚，其含量随产地差异较大，为生何首乌质量评价提供了可靠的指标和方法。 【关键词】何首乌 蒽醌 HPLC法 【中图分类号】R284【文献标识码】A【文章编号】2095-1752（2012）36-0131-03 Determination of anthraquinoid compounds in polygonum multiflorum produced in different areas by HPLC Wenjia,Pan*;Shuang, Lin; Wenmin, Li; Jiantao, Lin Guangdong medical college,dongguan, 523808 *:corresponding author 【Abstract】Objective: To determine the contents of anthraquinone before and after hydrolysis in the commercial medicinal materials of Radix Polygomi Multiflori collected from different areas respectively by RP-HPLC. Methods: The determination was carried out using C18 column，with mobile phase consisted of methanol-0.1% phosphoric acid solution(85:15),the detection wavelength was 254 nm. Results: Anthraquinone contents in Sichuan Emei Mountain sample is maximal.The free type and union type emodin contents were 235.59mg/100g，18.17mg/100g and the free type physcion was 97.69 mg/100g .The contents in the Chongqing Jin Yun county , Guizhou Shi Bing county, Guangxi Tian Yang sample were lower.Conclusion: Anthraquinone contained in the samples were mainly emodin and physcion.The contents varied greatly in the samples from the different producing areas. Make a Radix Polygomi Multiflori quality evaluation has provided the reliable index and method. 【Key words】polygonum multiflorum anthraquinone HPLC 何首乌始载于《开宝本草》，为蓼科植物何首乌Poamp;gonum mul tiflorum Thunb.的干燥块根。该植物为多年生缠绕性草本，块根多呈纺锤形，表面红棕色至暗褐色，断面紫红色，故又称为赤首乌[1]。其性微温，味苦、甘、涩。主要含有蒽醌类、二苯乙烯苷类和磷脂类等化学成分[2]。生首乌能解毒、消痈、润肠通便，治瘰疡疮痈、风疹瘙痒、肠燥便秘[3]。临床主要用于调血脂、强心、抗菌、抗癌、提高免疫功能等作用[4]。 何首乌的种植分布广泛,主产于河南、湖北、广西、广东、贵州、四川、江西等