antisense oligonucleotide against clusterin regulates human hepatocellular carcinoma invasion through transcriptional regulation of matrix metalloproteinase-2 and e-cadherin反义寡核苷酸对clusterin调节人类肝细胞癌侵犯通过矩阵metalloproteinase-2和钙粘蛋白的转录调控.pdfVIP

Int. J. Mol. Sci. 2012, 13, 10594-10607; doi:10.3390/ijms130810594 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Antisense Oligonucleotide Against Clusterin Regulates Human Hepatocellular Carcinoma Invasion Through Transcriptional Regulation of Matrix Metalloproteinase-2 and E-Cadherin Dong Chen 1,†, Yan Wang 2,†, Kejun Zhang 1, Xuelong Jiao 1, Bomin Yan 3 and Jun Liang 3,* 1 Department of General Surgery, the Affiliated Hospital of Qingdao Medical College, Qingdao University, Qingdao 266003, China; E-Mails: chendong.sdqd@ (D.C.); wlsdermyy@163.com (K.Z.); Jiaoxuelong@163.com (X.J.) 2 Department of Endodontics, School of Stomatology, Shandong University, Jinan 250012, China; E-Mail:wangyan1965@ 3 Department of Oncology, the Affiliated Hospital of medical college, QingDao University, QingDao 266003, China; E-Mail: Yanbm818@ † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: qyfyliang@163.com; Tel.: +86-532-8291-0257; Fax: +86-532-8291-1805. Received: 21 June 2012; in revised form: 10 August 2012 / Accepted: 20 August 2012 / Published: 23 August 2012 Abstract: Secreted clusterin (sCLU) has been shown to be overexpressed in metastatic hepatocellular carcinoma (HCC) tissue, and its overexpression in HCC cells increases cell migration and the formation of liver metastatic tumor nodules in vivo. In this study, we tested the hypothesis