analysis of mice lacking dnasei hypersensitive sites at the 5′ end of the igh locus分析小鼠缺乏dnasei高度敏感网站本5u2032末端的轨迹.pdfVIP

Analysis of Mice Lacking DNaseI Hypersensitive Sites at the 59 End of the IgH Locus 1.¤a 2.¤b 3 1¤c 2 Thomas Perlot , Inka Pawlitzky , John P. Manis , Ali A. Zarrin , Peter H. Brodeur , Frederick W. Alt1* 1The Howard Hughes Medical Institute, The Children’s Hospital, Immune Disease Institute, and Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America, 2 Immunology Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America, 3 Children’s Hospital Boston and Joint Program in Transfusion Medicine, Harvard Medical School, Boston, Massachusetts, United States of America Abstract The 5 9 end of the IgH locus contains a cluster of DNaseI hypersensitive sites, one of which (HS1) was shown to be pro-B cell specific and to contain binding sites for the transcription factors PU.1, E2A, and Pax5. These data as well as the location of the hypersensitive sites at the 5 9 border of the IgH locus suggested a possible regulatory function for these elements with respect to the IgH locus. To test this notion, we generated mice carrying targeted deletions of either the pro-B cell specific site HS1 or the whole cluster of DNaseI hypersensitive sites. Lymphocytes carrying these deletions appear to undergo normal development, and mutant B cells do not exhibit any obvious defects in V(D)J recombination, allelic exclusion, or class switch recombination. We conclude that deletion of these DNaseI hypersensitive sites does not have an obvious impact on the IgH locus or B cell development. Citation: Perlot T, Pawlitzky I, Manis JP, Zarrin AA, Brodeur PH, et al. (2010) Anal