analysis of microsatellite polymorphism in inbred knockout mice在纯系基因敲除小鼠的微卫星多态性分析.pdfVIP

Analysis of Microsatellite Polymorphism in Inbred Knockout Mice 1. 1. 2 2 1 1 1 Baofen Zuo , Xiaoyan Du , Jing Zhao , Huixin Yang , Chao Wang *, Yanhua Wu , Jing Lu , 1 1 Ying Wang , Zhenwen Chen * 1 Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, China, 2 Model Animal Research Center, Nanjing University, Nanjing, China Abstract Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this study, we further examined whether CMP also relates to the homologous recombination in gene knockout (KO) mouse strains. The same 42 microsatellite loci were analyzed by polymerase chain reaction (PCR) in 29 KO inbred mouse strains via short tandem sequence repeat (STR) scanning and direct sequence cloning to justify microsatellite polymorphisms. The C57BL/6J and 129 mouse strains, from which these 29 KO mice were derived, were chosen as the background controls. The results indicated that 10 out of 42 (23.8%) loci showed CMP in some of these mouse strains. Except for the trinucleotide repeat locus of D3Mit22, which had microsatellite CMP in strain number 9, the core sequences of the remaining 41 loci were dinucleotide repeats, and 9 out of 41 (21.95%) showed CMPs among detected mouse strains. However, 11 out of 29 (37.9%) KO mice strains were recognized as having CMPs. The popular dinucleotide motifs in CMP were (TG)n (50%, 2/4), followed by (GT)n (27.27%,