多穗柯总黄酮提取工艺及其含量动态变化研究.pdfVIP

※工艺技术 目 晶科字 2008,Vo1．29,No．06 139 多穗柯总黄酮提取工艺及其含量动态变化研究 李胜华1，伍贤进1，郁建平2，李思源1，韩永光· (1．湖南怀化学院生物工程系，湖南怀化 418008； 2．贵州大学生化营养研究所，贵州贵阳 550025) 摘 要：目的：探讨多穗柯总黄酮的提取工艺及含量动态变化。方法：以怀化产多穗柯为原料，采取乙醇为溶 剂超声波辅助提取，以L，(34)正交试验方法优化总黄酮提取工艺，然后在该工艺条件下对自然存放时间不同、采 摘季节不同、不同叶的多穗柯原料进行总黄酮含量的动态变化考察。结果：乙醇体积分数对总黄酮提取量有显著 影响，提取温度对总黄酮提取量有影响；多穗柯自然存放时间越长，总黄酮含量越低，平均每年下降24．74％； 不同季节采摘的多穗柯其总黄酮含量以 8～l0月最高；经对怀化多穗柯嫩叶和老叶中总黄酮的提取测定表明，多 穗柯中嫩叶总黄酮含量最高，二年生最低。结论：以原料35倍量的70％乙醇作为溶剂，在提取功率600W、提取 时间l5min的条件下，即可获得多穗柯总黄酮最佳提取效果；8～10月采摘嫩叶存放时间越短，总黄酮含量越高。 关键词：多穗柯；黄酮类化合物；提取工艺；含量；动态变化 Study on Extraction Technics and Content Variety Trends of Total Flavonoids in Lithocarpus polystachyus Rehd． LI Sheng—hua ，wU Xian-jin ，YU Jian—ping ，LI Si—yuan ，HAN Yong—guang (1．Department of Bioengineering，Huaihua College，Huaihua 418008，China； 2．Institution of Biochemistry and Nutrition，Guizhou Unive~ity，Guiyang 550025，China) Abstract：Object：To study extraction technics and variety trends of the total flavonoids in Lithocarpus polystachyus Rehd． Method：Using design of L9(3 )，the extraction conditions of total flavonoids from Lithocarpus polystachyus Rehd．in Huaihua were optimized by ethanol solution extraction assisted with ultrasonic，and the variety trends of total flavonoids content were investigated with different natural storage time，different gathering seasons，differrent Lithocarpus polystachyus Rehd．1eaves． Result：Extraction temperature had effect on total flavonoids yield，and ethanol concentration had more obvious effect than extraction temperature．The more natural storage time，the less total flavonoids content，and the average descent rate Was 24．74％ per year．Th e flavonoids content from Lithocarpus polyst