强骨饮对去卵巢大鼠腰椎骨形态计量学的影响.pdfVIP

中国骨伤2008年4月第21卷第4期 Ghina J Orthop＆Trauma，Apr．2008，Vo1．21，No．4 ·287· · 基 础 研 究 · 强骨饮对去卵巢大鼠腰椎骨形态计量学的影响 史晓林，昊连国，徐建春 (浙江中医药大学附属第二医院骨科，浙江 杭州 310005) 【摘要】 目的：观察自拟强骨饮对骨质疏松症的骨形态计量学的影响，并探讨其机制。方法：选健康雌性SD大鼠 60只，体重230~280 g。随机分为3组，强骨饮组(治疗组)、密钙息组(对照组)和空白组，每组20只。分别采取切除双 侧卵巢方法进行骨质疏松造模，10周造模成功后，开始给药，治疗组用自拟强骨饮灌胃，每日1次，每次0．001 ml／kg； 时熙组用密钙息皮下注射，每日1次，每次0．72U／kg。空白组，不做处理。在给药后45、90、135及 180d每组各取5只 大鼠进行检测，先测量体重，然后空气栓塞处死，分离腰椎，获得腰椎样本，经切片等处理后，显微镜下作骨形态计量学 检耐(包括骨体积分数、骨小梁厚度、骨小梁间距)。结果：给药后135d治疗组骨小梁厚度与对照组相比差异有统计学 意义。给药后18od治疗组骨小梁厚度、骨小梁间距、骨体积分数与对照组相比均具有统计学意义。治疗组与空白组相 比骨小粱厚度 骨小梁问距、骨体积分数差异均具有统计学意义。结论：自拟强骨饮可以明显改善骨形态计量学指标， 可能是通过刺激成骨细胞生长，抑制破骨细胞活性，并抑制高骨转换趋势来实现的。 【关■词】腰椎；骨质疏松，绝经后； 骨生成； 中药疗法 EffectofQhu~gudrinkf强骨饮)onbonehistomorphometryoflumbarvertebraeinovariectomizedrats SHIXiao-lin， U Lian-guo。XU Jian-chun．The Second Affiliated Hospital ofZhejiang Traditional Chinese Medicine University，Hangzhou 31oo∞，Zl~jiang，China M$STl CT Objective：To observe the effect ofChinese herbal medicine Q／~gga drink(强骨饮)on bone histomorphome- try of lumbar vertebrae in ovariectomized rats and to explore its mechanism．Methods：Sixty female SD rats(weight 230 to 280 gram)were randomizedintothree groups：Qianggudrink(强骨饮)group(treatment group)，Miacalcic group(control group)and blank group．The osteoporosis model was established successfully after all rats were OVariectomized for 10 weeks．The treatment group WaS used intragastric infusion with Qianggn drink(强骨饮)0．001 ml／kg once a day．The control group was used hypo- dermic injection once a day with Miacalcic 0．72 U／kg．Five rats were killed respectively and the lumbar specimens were taken andcutfor bone histomorphometric analysis at 45，90，135 and 180 days afterthe treatment．Results：The thickness oftrabecu． 1ar bone in treatment group had the statistical significance with control group at 135 days after ovariectomized rats were