星点设计效应面法优选白芷中欧前胡素和异欧前胡素的醇提工艺.pdfVIP

年第年第 2828 卷第卷第 22 期期 ·制 剂 工 艺 星点设计效应面法优选白芷中欧前胡素 和异欧前胡素的醇提工艺 李 靖，路莎莎 张掖市中医医院，甘肃 张掖 734000 ［摘 要］目的：利用效应面法优选白芷的醇提取工艺参数。 方法：采用 HPLC 方法，Waters 公司的 Sun- fire C18 色谱柱（150 mm×4.6mm，5 μm）；流动相为甲醇- 水（70∶30），等度洗脱；流速 0.9 mL/min；检测波长 254 nm；柱温 30℃；分析时间为 20 分钟；进样量 10 μL。结果：白芷中欧前胡素的最佳提取工艺为甲醇浓度 83.17%， 提取时间 9.15 小时，甲醇用量为 147.32 mL；异欧前胡素的最佳提取工艺为甲醇浓度 91.83%，提取时间 9.15 小时，甲醇用量为 112.68 mL。 结论：采用星点设计与效应面法相结合的方法对实验工艺优化更方便、更准确。 ［关键词］白芷；欧前胡素；异欧前胡素；星点设计；效应面法 ［中图分类号］R259 ［文献标识码］A ［文章编号］1004-6852(2015)02-0023-04 Alcohol Extraction of Imperatorin and Isoimperatorin from Selected Baizhi by CCD and RSM LI Jing,LU Shasha Zhangye Hospital of Chinese Medicine, Zhangye 734000, China Objective: To collect the extracting parameters of the alcohol from Baizhi (angelica dahurica) by central composite design (CCD) and response surface method (RSM). Methods: The HPLC method was performed and the detected conditions were defined, chromatography column: Waters Sunfire C 18 (150 mm×4.6mm, 5 μm); mobile phase: CHOH/H2O = 70/30, isocratic elution; flow rate: 0.9 mL/min; detection wavelength: 254 nm; column temperature: 30℃; analyzing time: 20 min; injection volume: 10 μL. Results: The best concentration of methanol for extracting the imperatorin from Baizhi was 83.17%, the extracting time was 9.15 hours and the methanol dosage was 147.32 mL，while for extracting the isoimperatorin the best concentration of methanol was 91.83% with 9.15 extract- ing hours and 112.68 mL methanol dosages. Conclusion: The method of combining CCD and RSM is easier and more