介绍一种新的家兔室性心律失常模型.docVIP

精品论文 参考文献 介绍一种新的家兔室性心律失常模型 冯建芳 杜景霞 李艳 (通讯作者) 　　(河南科技大学 河南洛阳 471003) 　　【摘要】目的：观察不同浓度水合氯醛对家兔心电图的影响，建立适合本科教学的新的家兔室性心律失常模型。方法：采用家兔心电图描记方法,将健康家兔分为6组：水合氯醛低剂量组（5%，0.4 ml/kg）、水合氯醛中剂量组（10%，0.4 ml/kg）、水合氯醛高剂量组（15%，0.4 ml/kg）、水合氯醛高剂量+利多卡因组(1%,1ml/kg)、氯化钡组（0.5%，2ml/kg）、氯化钡+利多卡因组。比较各组心律失常发生率和持续时间。结果：水合氯醛导致家兔室性心律失常持续时间超过30min，与氯化钡组比较死亡率低（p＜0.001）；利多卡因可明显缩短水合氯醛室性心律失常持续时间（p＜0.05），心电图可恢复正常，与氯化钡组比较（p＜0.05）。结论：15%水合氯醛0.4ml/kg可以引起家兔稳定的室性心律失常，利多卡因有对抗作用。该模型与氯化钡模型比较具有操作简单，毒性小，心电图稳定，动物死亡率低等优点，更适合本科教学需要。 　　【关键词】家兔 水合氯醛 氯化钡 心律失常模型 　　【中图分类号】R3 【文献标识码】A 【文章编号】1672-5085（2014）18-0284-02 　　A New Model of Rabbit Ventricular Arrhythmia 　　FENG Jian-fang,DU Jing-xia,LI Yan 　　Henan university of science and technology,luoyang 471003 　　【Abstract】Objective:To observe the influence of different concentration of chloral hydrate in electrocardiogram (ECG) on rabbit, establish a new model of rabbit ventricular arrhythmia suitable for undergraduate teaching. Methods: Rabbit electrocardiogram (ECG) trace method, healthy rabbit can be divided into 6 groups, Chloral hydrate low dose group（5%，0.4 ml/kg）, Chloral hydrate middle dose group（10%，0.4 ml/kg）, Chloral hydrate high dose group（15%，0.4 ml/kg）, high dose of chloral hydrate and lidocaine(1%,1ml/kg), barium-chloride(0.5%,2ml/kg), barium- chloride and lidocaine group. The incidence of arrhythmia and duration of each group was compared.Results: High dose group of arrhythmia duration more than 30 minutes, Compared with barium chloride group mortality was lower (p lt; 0.001); To compared with barium chloride group，lidocaine can obviously shorten the duration of the chloral hydrate ventricular arrhythmia (p lt; 0.05), electrocardiogram (ECG) return to normal (p lt; 0.05). Conclusion: 15% chloral hydrate 0.4 ml/kg can cause stability on rabbit ventricular arrhythmia, Lidocaine against arrhythmia caused by chloral hydrate ventricular arrhythmia on rabbit. Compared with barium chloride model it is simple