中枢神经系统星型细胞肿瘤中MGMT,Ki67 表达与相关性研究.docVIP

精品论文 参考文献 中枢神经系统星型细胞肿瘤中MGMT,Ki67 表达与相关性研究 郭欣茹 （海军总医院，全军神经外科研究所，北京，100048） [摘要]：目的：探讨MGMT,Ki67 在中枢神经系统星型细胞肿瘤组织中的表达及其与肿瘤恶性程度之间的相关性及其意义。方法：应用免疫组化方法检测不同 级别80 例人脑中枢神经系统星型细胞瘤组织与15 例正常人脑组织中MGMT,Ki-67 蛋白的表达情况及相关性。结果：MGMT,Ki-67 经蛋白免疫组织化学染色后在 各级别星型细胞瘤标本中均有阳性表达并随着肿瘤病理级别的升高，表达强度增加。结论：在各级别星型细胞瘤中,MGMT 和Ki-67 蛋白表达的阳性细胞百分比呈 逐渐升高的趋势,且在正常脑组织均为阴性，提示MGMT 和Ki-67 蛋白在人脑中枢神经系统星型细胞瘤中的表达与脑中枢神经系统星型细胞瘤的恶性程度、组织分 化有关。判断肿瘤的浸润和转移，预测脑中枢神经系???星型细胞瘤恶性程度以及预后具有。 [关键词]：中枢神经系统星型细胞瘤； MTMG； Ki-67 The Interdependency between the Expression of MGMT，Ki-67 in Astrocytoma of Central Nervous System GUO Xinru ( The Navy General hospital of PLA , Neurosurgical Institute of PLA, Beijing,100048) [ABSTRACT]OBJECTIVE: This paper is want to find the interdependency and the importance between the expression of MGMT, ki67, CD34 in Astrocytoma and the pathological grading of tumor. METHODS: Immunohistochemistry was used to detect MGMT, Ki-67 protein expression and correlation in different levels of 80 cases of human Astrocytoma tissues and 15 cases of normal brain tissue. RESULTS: MGMT, Ki-67 protein by immunohistochemical staining in each grade Astrocytoma specimens were positive expression and with histological grade elevated expression intensity also increased. CONCLUSION: At all levels Astrocytoma, the percentage of MGMT and Ki-67 protein expression in positive cells was gradually increased, and in normal brain tissue were negative, which points out that the expression of MGMT and ki-67 in human brain Astrocytomas has related to the malignant degree of Astrocytomas and tissue differentiation. Therefore, the mark for determining tumor invasion and metastasis, also for Malignant Astrocytoma predict and prognosis has certain significance. [KEYWORDS]: Astrocytomas; MGMT; Ki-67 人中枢神经系统星型细胞瘤是神经系统中最常见且侵袭性最强 的恶性肿瘤，严重影响人类健康。目前有手术、放疗、化疗或其组 合的综合治疗措施。迄今为止，手术切除后进行化疗仍是综合治疗 措施中重要步骤之一，但化疗后疗效欠佳，患者的预后并无明显的 改善。[1] 肿瘤对化疗药物的耐受是影响化疗效果的重要因素之一， DNA 修复酶:O6-甲基鸟嘌呤