两种生物指示剂对压力蒸汽灭菌器生物监测的影响.docVIP

精品论文 参考文献 两种生物指示剂对压力蒸汽灭菌器生物监测的影响 安玉梅 周存英 朱友慧（山东省日照市东港区人民医院 276800） 【摘 要】 目的 探讨用嗜热脂肪杆菌芽孢制成的菌片和自含式生物指示剂对生物监测的影响。方法 采取观察组和对照组，对照组采用嗜热脂肪杆菌芽孢制成的菌片+自配的培养基（溴甲酚紫葡萄糖蛋白胨）对压力蒸汽灭菌器进行生物监测；观察组采用自含式生物指示剂对压力蒸汽灭菌器进行生物监测，并将两种方法所产生的结果进行比较。结果 观察组生物监测合格率达100﹪，对照组生物监测的合格率达67﹪，两组结果比较有差异（P﹤0.05）,且观察组培养结果提前6 天。结论 生物监测采用自含式生物指示剂，更快、更直观的观察到结果，而且生物监测测合格率高，更好的保证无菌物品的质量，确保无菌物品合格率达100﹪，值得在供应室推广使用。 【关键词】 生物指示剂；压力蒸汽灭菌器；生物监测 Two living thing indicator to pressure steam sterilizer biology monitoring result influence. AN Yu—mei, ZHOU Cun—ying, LIU Xiang—juan. Rizhao Donggang District Peoples Hospital sunshine, Shandong 276800 China. [Abstract] Objective To investigate stearothermophilus Bacillus bacteria piece made and self-contained biological indicator for biological monitoring. Methods Take the observation group and control group, the control group made stearo thermophilus Bacillus bacteria + medium (bromocresol purple glucose peptone) with the right pressure steam sterilizer for biological monitoring; the observation group were treated with self-contained biological indicator for pressure steam sterilization, biological monitoring, and the two methods compare the results. Results Pass rate of 100%, control 67 percent of the qualified rate of the group of biological monitoring observation group biological monitoring results, two sets of results are different (P lt;0.05), and the observation group culture six days in advance. Conclusion Biological monitoring using self-contained biological indicator, faster, more intuitive observed results and biological monitoring measured high pass rate, a better guarantee the quality of the sterile, ensure sterile 100% pass rate, it is worth promote the use of the supply room. [Keywords] Biological indicators; Pressure steam sterilizer; Biological monitoring 2009 年1～12 月，我院供应室应用自含式生物指示剂进行生物监测，同期使用嗜热脂肪杆菌芽孢制成的菌片进行生物监测，在培养周期、培养结果等方面进行比较，以探讨不同生物指示剂对压力蒸汽灭菌器生物监测的影响，现报告如下： 1 资料与方法 1.1 观察组用240 个自含式生物指示剂试管进行试验，设96 个对照管；对照组用2