两种组织工程支架性能的实验研究.docVIP

精品论文 参考文献 两种组织工程支架性能的实验研究 张蕊1 柏国艳1 赵彬2(通讯作者) 张芳3 (1山西医科大学 030001;2山西医科大学口腔医院修复科 030001;3山西医科大学口腔医学系 030001) 【中图分类号】R68 【文献标识码】A【文章编号】1672-5085(2013)11-0427-02 【摘要】 目的 对两种组织工程支架的性能进行研究，为寻求更符合组织工程的支架提供实验依据。方法 采用二次冻干技术制备CS-SF-TCP及CS支架，采用液体替代法等方法对两种支架的一般性能进行测定，将制备成一定体积的两种支架植入Wistar大鼠的背部，于2、4、6、周取出植入物行组织学染色观察。结果 制备的两种支架成白色海绵状物质，孔隙率为70%-80%，孔径值为100-150micro;m。埋植支架周围皮下组织无充血、化脓等现象,无组织坏死发生,植入部位早期有轻度的炎症反应,后较快消退。植入物表变均被致密的薄层纤维膜包绕。组织染色结果可见支架的表面有细胞附着，两种支架内部孔隙失去连接，有断裂吸收现象。结论 单纯CS支架在体内降解过快，CS-SF-TCP支架具有良好的降解性与组织相容性,是一种很有潜力的组织工程支架材料。 【关键词】 组织工程 丝素 壳聚糖 支架 The experimental study of property about two kinds of tissue engineering scaffolds ZHANG Rui ZHAO Bin BAI Guo-yan ZHANG Fang Department Stomatology of Shanxi Medical university Taiyuan 030001 china 【Abstract】PURPOSE: The purpose of the study is to research the property about the chitosan scaffold and the chitosan-silk fibroin-tricalcium scaffold, in order to find the more suitable scaffold for tissue engineering. METHODS: The two scaffolds were prepared by freezing and lyophilizing taking water as the hole-making agent. The two scaffolds phosphate complex was implanted in the dorsal muscles of rats for assessment of in vitro and in vivo degradation as well as biocompatibility. RESULTS: These scaffolds phosphate complex was spongy, with uniform porosity and irregular shapes of pores. The pores were connected with each other, with a porosity of 70%-80% and diameter of approximately 100-150mu;m. All the implants were covered by thick memberance. These scaffolds were loss the connect. Conclusion The chitosan-silk fibroin-tricalcium phosphate porous complex was more appropriate as the tissue engineering scaffold. 【Key words】Tissue engineering Silk fibroin Chitosan Scaffold 前言 骨组织工程的兴起为临床上各类骨缺损的修复提供了良好的思路。三维多孔支架是骨组织工程的重要基础，它不仅起到支撑，保持原有组织形状的作用，而且还为种子细胞提供生长，分化的空间。从而引导缺损组织的再生，起到“桥梁”的作用。 磷酸三钙类似于人骨的天然无机构成, 植入后与机体骨接合良好，骨传导性好且具有良好的生物相容性及生物降解性,是目前应用于骨组织工程最普遍的材料。但其生物强度差，受冲击能力弱，不能用于负荷部位的骨缺损。另外它的降解速率与新骨