离子色谱法测定低钠盐中有机酸的含量.pdfVIP

2014 年第 1 期 Vol.24 No. 1 检验检疫学刊 JOURNAL OF INSPECTION AND QUARANTINE 离子色谱法测定低钠盐中有机酸的含量 陈建华 陈 魏 胡德聪 （湖北出入境检验检疫局湖北武汉 430050 ） 摘要摘要 建立了一种用离子色谱法同时测定低钠盐样品中苹果酸、酒石酸和柠檬酸等有机酸的分析方法。 样品经溶解、过滤、净化后，再经IonPac AS11色谱柱分离，以自动淋洗液发生装置生成20 mmol/L-40 mmol/L 的KOH进行梯度洗脱，抑制电导检测器检测。3种有机酸在2.5 mg/L-50 mg/L 范围内呈现良好的线性，相关系 数均大于0.998 ；加标回收率为76.0%-98.2%、相对标准偏差小于10%；3种有机酸的检出限均为0.025% 。方法 回收率好、精密度高、检出限低、可操作性强，可满足低钠盐中有机酸组分的测定。 关键词关键词 低钠盐；有机酸；离子色谱法 中图分类号中图分类号 O657.7 Determination of Organic Acid Content in Low Sodium Edible Salt with Ion Chromatography Chen Jianhua, Chen Wei, Hu Decong (Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan, Hubei, 430050) Abstract: A new method was developed to determine three kinds of organic acid, such as malic acid, tartaric acid and citric acid in low sodium edible salt with ion chromatography in this paper. Malic acid, tartaric acid and citric acid of the samples were separated by IonPac AS11 chromatographic column after the samples were dissolved, fi ltered and cleaned up. Three organic acids by ion chromatography was gradiently eluated by 20 mmol/L-40 mmol/L KOH and determined by suppressed conductivity. Three organic acids showed good liner relationship in 2.5 mg/L-50 mg/L, and the correlation coeffi cients were all more than 0.998. The recoveries of three organic acids for three level spiked samples ranged between 76.0% and 98.2%and the relative standard deviation was less than 10%, and the detection limits of three organic acids were 0.025%. The method showed a good recovery and precision, low detection limit and easy operation. It was therefore suitable for the determination of organic acid content in low sodium edible salt. Key Words: Low Sodium Edible Salt; Organic Acid; Ion Chromatography 1 前言