不同时间饮用普洱茶对消化功能影响实验探究.docVIP

不同时间饮用普洱茶对消化功能影响实验探究 　 作者：张琪瑶　余小倩　谭红梅 【摘要】 　　目的 观察不同时间饮用普洱茶对消化功能的影响，探求普洱茶促进消化道蠕动、调脂减肥的机制及最佳饮用时间。方法 本实验将100只小白鼠分设标准组、无茶组、同时加茶组、10min后加茶组及预实验组，实验结束时解剖观察，检测小鼠在餐时及餐后不同时间用普洱茶灌胃后的小肠推进度。运用SAS程序进行完全随机设计资料方差分析。结果 餐后10min饮用普洱茶的小肠推进度大于进餐同时饮用普洱茶的小肠推进度。鞣酸成分影响小肠推进度，对消化道蠕动功能有抑制作用。结论 研究提示含低鞣酸的普洱茶有明显促进消化道蠕动功能。餐后10min饮茶的调脂减肥保健作用优于进餐同时饮用的效果。 【关键词】 不同时间；普洱茶；小肠推进度；消化道蠕动；减肥 【Abstract】 Objective To observe the effect of taking puer tea in different time on digestive system, investigating the mechanism of puer tea in stimulating the digest system and reducing blood fat, studying the best time to take the tea. Methods 100 mice were divided into 6 groups, including (Ⅰ) standard group, taking protein and tannic acid at the same time, (Ⅱ) blank group, taking protein and water simultaneously, two experimental groups（Ⅲ）and (Ⅳ), and two pre-experiment groups(Ⅴ) and(Ⅵ) , one of which took tea and protein at the same time, while the other take tea 10minuts later.At the end of 20 minutes dissected the mice and study the intestinal movement of the groups that given puer tea and food simultaneously or separately. Use SAS system.Results The intestinal movement when taking puer tea ten minutes after diet is faster than taking the tea with food simultaneously. Conclusion It suggested that the puer tea with low level of tennic acid stimulate digest system obviously. The effect of taking puer tea ten minutes after diet on keeping fit is better than taking the tea with food simultaneously. 【Key words】 different time; puer tea; intestinal movement; digestive movement; keep fit 据我国古书记载及现代国内外研究报告，称为中国国粹的茶叶具有“调脂减肥保健”的作用。然而，餐后多长时间内饮用普洱茶才能达到最好的保健效果尚缺乏实验依据。本实验利用普洱茶叶中鞣酸成分对消化的抑制作用及茶中茶多酚对消化的促进作用，通过在不同时间发挥作用进行比较观察，检测不同时间饮用普洱茶对消化系统的影响，对最佳饮用时间作出较为准确的推测，从而进一步阐释普洱茶的药用价值。 1 材料与方法 1.1 动物与材料 小白鼠40只，体重约28kg,随机分为4组（标准组、无茶组、同时加茶组、10min后加茶组），每组10只。实验前断食不断水12h。胃肠内标记物：墨汁。药品：蛋白液0.3g/ml, 普洱茶0.06g/ml, 鞣酸(单宁酸)0.0039g/ml。 检测小肠推进度的方法：量取幽门括约肌至色素最前端及小肠全长的距离,以二者之比为小肠推进率。即：小肠推进率=（幽门至色素前沿的距离）/（幽门至回盲部）×100% 1.2 预实验 小白鼠60只。（1）测定小鼠消化周期