γ射线照射及非照射人肌腱生物力学比较探究.docVIP

γ射线照射及非照射人肌腱生物力学比较探究 　【摘要】 [目的]探讨目前国内外最普遍应用的“γ”射线照射消毒法对人肌腱生物力学的影响。[方法]将等长的24条肱二头肌肌腱长头配对分成两组，A组(12)：非照射组，B组(12)：经2.5Mrad“γ”射线照射组。采用WDW-3 020电子万能材料试验机测定结构力学和材料力学。[结果]经拉伸实验后肌腱平均长度B组为A组的99.15%，最大拉长量为93.46%，刚度为95.27%，最大载荷能量为93.60%,最大应力为84.88%,最大应变为85.82%,弹性模量为90.40%，能量密度为66.73%，B组照射组与A组非照组比较Plt;0.05,有统计学意义，照射后生物力学各参数明显降低。[结论]“γ”射线照射消毒后肌腱强度明显降低，是否采用“γ”射线照射消毒同种异体组织有待于进一步研究。 【关键词】 生物力学; 人; 交叉韧带; 移植; 同种异体; 照射; 肌腱 　　Abstract: [Objective]To explore the change of biomechanics about human tendon with“γ”irradiated which was universal used by domestic and foreign. [Methods]Twenty-four upper limb tendon of human with the same length were divided into two groups,group A(12):nonirradiated group, group B (12):2.5 Mrad gamma irradiated group.Material properties and structural properties were determined with WDW-3020 electron universal testing machine.[Results]The mean length of the tendon was 99.15% of control, the elongation to failure was 93.46%, the linear stiffness was 95.27%, the energy to maximum force was 93.60%, the maximum stress was 84.88%, the strain to failure was 85.82%, the linear modulus was 90.40%, the strain energy density to maximum was 66.73%. Difference was noted after compared hetween group A and B (Plt;0.05). All biomechanical parameter presented significantly decline after irradiated.[Conclusion]The tendon strength reduced obviously after irradiated, it needs further studies whether disinfect allograft could be irradiated with Gamma radiation on clinical. 　　Key words：biomechanics; human; cruciate ligament; transplant; allograft; irradiate; tendon 　　交叉韧带是膝关节稳定的主要结构，交叉韧带重建术是治疗交叉韧带损伤的主要方法[1]。自1978年Neviaser等人应用冻干保存的同种异体肩袖移植修复大范围的肩袖损伤，1981年Green等人将冷冻保存后的异体阔筋膜用于肌腱、韧带损伤的修复相继成功以来，人们开始了同种异体组织重建膝关节前交叉韧带的临床及基础研究。但同种异体组织移植仍有疾病传播、感染、松弛等诸多问题。为了防止艾滋病、肝炎等疾病的传播，国内外一些学者及组织探讨了一系列的消毒措施，目前最常用的是“γ”射线照射消毒。有研究表明，在2.0Mrad“γ”照射对同种异体组织的最大应力，最大应变等有一定负面影响[2]，本文对国内外普遍采用的2.5Mrad“γ”射线照射消毒法对肌腱生物力学指标的影响进行全面探讨。 　　1 材料与方法 　　1.1 实验材料准备 　　由解放军总医院第一附属医院组织库提供的24条等长肌腱配对分成A、B两组，每一对来自同一供体。A组为对照组，没有经过“γ”射