an evaluation of the performance and acceptability of three led fluorescent microscopes in zambia lessons learnt for scale-up评估性能和可接受性的三个领导荧光显微镜在赞比亚扩大的教训.pdfVIP

an evaluation of the performance and acceptability of three led fluorescent microscopes in zambia lessons learnt for scale-up评估性能和可接受性的三个领导荧光显微镜在赞比亚扩大的教训.pdf

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an evaluation of the performance and acceptability of three led fluorescent microscopes in zambia lessons learnt for scale-up评估性能和可接受性的三个领导荧光显微镜在赞比亚扩大的教训

An Evaluation of the Performance and Acceptability of Three LED Fluorescent Microscopes in Zambia: Lessons Learnt for Scale-Up 1,2 1 1,2 3 4 Eleanor R. Turnbull *, Kaunda Kaunda , Jennifer B. Harris , Nathan Kapata , Mweemba W. Muvwimi , Annika Kruuner1,2, German Henostroza1,2, Stewart E. Reid1,2 1 Centre for Infectious Disease Research in Zambia, Lusaka, Zambia, 2 Schools of Medicine and Public Health, University of Alabama at Birmingham, Birmingham, Alabama, United States of America, 3 National TB/Leprosy Program, Zambian Ministry of Health, Lusaka, Zambia, 4 Chest Diseases Laboratory/National Tuberculosis Reference Laboratory, Zambian Ministry of Health, Lusaka, Zambia Abstract The World Health Organization recommends the roll-out of light-emitting diode (LED) fluorescent microscopes (FM) as an alternative to light microscopes in resource-limited settings. We evaluated the acceptability and performance of three LED FMs after a short orientation among laboratory technicians from government health centers in Zambia. Sixteen technicians with varied light microscopy experience were oriented to FMs and divided into groups; each group read a different set of 40 slides on each LED FM (Primo Star iLEDTM, LuminTM, FluoLEDTM) and on a reference mercury-vapor FM (Olympus BX41TF). Slide reading times were recorded. An experienced FM technician examined each slide on the Olympus BX41TF. Sensitivity and specificity compared to TB culture were calculated. Misclassification compared to the experienced technician and inter- rater reliability between trainees was assessed. Trainees rated microscopes on technical aspects. Primo Star iLEDTM, FluoLEDTM and Olympus BX41TF had

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