an evaluation of putative sympatric speciation within limnanthes (limnanthaceae)评估中假定的同域物种形成limnanthes(limnanthaceae).pdfVIP
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an evaluation of putative sympatric speciation within limnanthes (limnanthaceae)评估中假定的同域物种形成limnanthes(limnanthaceae)
An Evaluation of Putative Sympatric Speciation within
Limnanthes (Limnanthaceae)
Stephen C. Meyers*, Aaron Liston, Robert Meinke
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, United States of America
Abstract
Limnanthes floccosa ssp. floccosa and L. floccosa ssp. grandiflora are two of five subspecies within Limnanthes floccosa
endemic to vernal pools in southern Oregon and northern California. Three seasons of monitoring natural populations have
quantified that L. floccosa ssp. grandiflora is always found growing sympatrically with L. floccosa ssp. floccosa and that their
flowering times overlap considerably. Despite their subspecific rank within the same species crossing experiments have
confirmed that their F1 hybrids are sterile. An analysis of twelve microsatellite markers, with unique alleles in each taxon,
also shows exceedingly low levels of gene flow between populations of the two subspecies. Due to the lack of previous
phylogenetic resolution among L. floccosa subspecies, we used Illumina next generation sequencing to identify single
nucleotide polymorphisms from genomic DNA libraries of L. floccosa ssp. floccosa and L. floccosa ssp. grandiflora. These data
were used to identify single nucleotide polymorphisms in the chloroplast, mitochondrial, and nuclear genomes. From these
variable loci, a total of 2772 bp was obtained using Sanger sequencing of ten individuals representing all subspecies of L.
floccosa and an outgroup. The resulting phylogenetic reconstruction was fully resolved. Our results indicate that although L.
floccosa ssp. floccosa and L. floccosa ssp. grandiflora are closely related, they are not sister taxa and therefore likely did not
diverge as a result of a sympatric speciation event.
Citation: Meyers SC, Liston A, Meinke R (2012) An Evaluation of Putati
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