adhesion forces and coaggregation between vaginal staphylococci and lactobacilli粘附力和阴道之间coaggregation葡萄球菌和乳酸杆菌.pdfVIP

Adhesion Forces and Coaggregation between Vaginal Staphylococci and Lactobacilli 1,2 2 3 2 1,4 Jessica A. Younes , Henny C. van der Mei *, Edwin van den Heuvel , Henk J. Busscher , Gregor Reid 1 Human Microbiology and Probiotics, Lawson Health Research Institute, London, Ontario, Canada, 2 Department of BioMedical Engineering, W. J. Kolff Institute, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands, 3 Department of Epidemiology, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands, 4 Departments of Microbiology Immunology, and Surgery, University of Western Ontario, London, Ontario, Canada Abstract Urogenital infections are the most common ailments afflicting women. They are treated with dated antimicrobials whose efficacy is diminishing. The process of infection involves pathogen adhesion and displacement of indigenous Lactobacillus crispatus and Lactobacillus jensenii. An alternative therapeutic approach to antimicrobial therapy is to reestablish lactobacilli in this microbiome through probiotic administration. We hypothesized that lactobacilli displaying strong adhesion forces with pathogens would facilitate coaggregation between the two strains, ultimately explaining the elimination of pathogens seen in vivo. Using atomic force microscopy, we found that adhesion forces between lactobacilli and three virulent toxic shock syndrome toxin 1-producing Staphylococcus aureus strains, were significantly stronger (2.2–6.4 nN) than between staphylococcal pairs (2.2–3.4 nN), especially for the probiotic Lactobacillus reuteri RC-14 (4.0–6.4 nN) after 120 s of bond- strengthening. Moreover, stronger adhesion