absence of xmrv and closely related viruses in primary prostate cancer tissues used to derive the xmrv-infected cell line 22rv1缺乏xmrv和密切相关的病毒在原发性前列腺癌组织中用于推导出22 rv1 xmrv-infected细胞系.pdfVIP

Absence of XMRV and Closely Related Viruses in Primary Prostate Cancer Tissues Used to Derive the XMRV- Infected Cell Line 22Rv1 1 2 3 1 4 5 Jaydip Das Gupta , Ka-Cheung Luk , Ning Tang , Christina Gaughan , Eric A. Klein , Eugene S. Kandel , 2 1 John Hackett Jr , Robert H. Silverman * 1 Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, 2 Abbott Diagnostics, Emerging Pathogens and Virus Discovery, Abbott Park, Illinois, United States of America, 3 Abbott Molecular, Des Plaines, Illinois, United States of America, 4 Glickman Urologic and Kidney Institute, Cleveland Clinic, Cleveland, Ohio, United States of America, 5 Roswell Park Cancer Institute, Buffalo, New York, United States of America Abstract The 22Rv1 cell line is widely used for prostate cancer research and other studies throughout the world. These cells were established from a human prostate tumor, CWR22, that was serially passaged in nude mice and selected for androgen independence. The 22Rv1 cells are known to produce high titers of xenotropic murine leukemia virus-related virus (XMRV). Recent studies suggested that XMRV was inadvertently created in the 1990’s when two murine leukemia virus (MLV) genomes (pre-XMRV1 and pre-XMRV-2) recombined during passaging of the CWR22 tumor in mice. The conclusion that XMRV originated from mice and not the patient was based partly on the failure to detect XMRV in early CWR22 xenografts. While that deduction is certainly justified, we examined the possibility that a closely related virus could have been present in primary tumor tissue. Here we report that we have located the original prostate tu