adipose inflammation initiates recruitment of leukocytes to mouse femoral artery role of adipo-vascular axis in chronic inflammation股动脉脂肪炎症启动招募白细胞鼠标adipo-vascular轴在慢性炎症的作用.pdfVIP

Adipose Inflammation Initiates Recruitment of Leukocytes to Mouse Femoral Artery: Role of Adipo- Vascular Axis in Chronic Inflammation 1,2,3 1 2 1 Sumihiko Hagita , Mizuko Osaka , Kentaro Shimokado , Masayuki Yoshida * 1 Life Science and Bioethics Research Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan, 2 Department of Vascular Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan, 3 Research Fellow of Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo, Japan Abstract Background: Although inflammation within adipose tissues is known to play a role in metabolic syndrome, the causative connection between inflamed adipose tissue and atherosclerosis is not fully understood. In the present study, we examined the direct effects of adipose tissue on macro-vascular inflammation using intravital microscopic analysis of the femoral artery after adipose tissue transplantation. Methods and Results: We obtained subcutaneous (SQ) and visceral (VIS) adipose tissues from C57BL/6 mice fed normal chow (NC) or a high fat diet (HF), then transplanted the tissues into the perivascular area of the femoral artery of recipient C57/BL6 mice. Quantitative intravital microscopic analysis revealed an increase in adherent leukocytes after adipose tissue transplantation, with VIS found to induce significantly more leukocyte accumulation as compared to SQ. Moreover, adipose tissues from HF fed mice showed significantly more adhesion to the femoral artery. Simultaneous flow cytometry demonstrated upregulation of CD11b on peripheral granulocyte and monocytes after adipose tissue transplantation. We also observed dominant expressions of the inflammatory cytokine IL-6, and chemokines MCP-1 and MI