塞来昔布对人雄激素非依赖性前列腺癌PC-3细胞体外作用和其机制得研究.pdf

中文摘要 2塞来昔布可影响人前列腺癌PC．3细胞的细胞周期。随着药物浓度 的增大，PC．3细胞Go／Gl期细胞数明显增多，S和G2／M期细胞数减少， 细胞周期阻滞于Go／G1期。 、 3塞来昔布可以诱导人前列腺癌PC．3细胞凋亡。随着药物浓度的增 大，PC．3细胞发生凋亡增加，凋亡率逐渐升高，差异有显著性。 塞来昔布浓度的增大，Caspase．3表达逐渐增多，呈浓度依赖性。推测激 活Caspase．3是塞来昔布诱导PC．3细胞凋亡的机制之一。 关键词：塞来昔布；Caspase一3；前列腺癌；增殖；凋亡 英文摘要 ofEffectsand Study MechanismsofCelecoxibonProlifertion and inHH — uman CancercancerPC．3CellsellsInInVitro Apoptosis prostate Apoptosis ABSTRACT canceriSoneofthecommoncancersinoldermen objective：Prostate withan incidence．At and is increasing present，incidencemortalityincreasing disease onthe of most yearbyyear．This dependspresenceandrogen，and with effective ofcastrationor treatments combined patientsearly androgen few aftera monthstoseveralcancerisoftentransformed blockade，but years into isoneofthemost hormone-refractorycancer(HRPC)．HRPC prostate issuesin theworldthereisnoeffective complex urologicaloncology,and treatmentnow．TreatmentofHRPChasbecomethebottleneckof Urology． is neededthat moreeffectivemethodsand Therefore，it urgently exploring treatmentsto the and life．Athomeand of improvepatient’Sprognosisquality abroadstudiesinrecenthavefoundedthatCOX-2is in