molecular-phylogenetic characterization of the microbiota in ulcerated and non-ulcerated regions in the patients with crohns diseasemolecular-phylogenetic表征微生物群的溃烂和non-ulcerated地区在克罗恩病的患者.pdfVIP

Molecular-Phylogenetic Characterization of the Microbiota in Ulcerated and Non-Ulcerated Regions in the Patients with Crohn’s Disease 1,2 1,2 1 1,2 1,2 Qiurong Li , Chenyang Wang , Chun Tang , Ning Li , Jieshou Li * 1 Research Institute of General Surgery, Jinling Hospital, Nanjing, Jiangsu, People’s Republic of China, 2 Department of Surgery, School of Medicine, Nanjing University, Nanjing, Jiangsu, People’s Republic of China Abstract Background: The dysbiosis of intestinal microbiota has been established in Crohn’s disease (CD), but the molecular characterization of this dysbiosis in Chinese subjects with CD remains unclear. This study aims to investigate the predominant bacterial composition of the faecal and mucosal-associated microbiota in Chinese CD patients using culture- independent techniques. Methods/Principal Findings: Eighteen patients with CD and 9 healthy controls were included in this study. The faeces and the intestinal mucosal tissues from the ulcerated and nonulcerated sites were subjected to bacterial community fingerprinting using denaturing gradient gel electrophoresis (DGGE). The predominant bacterial composition in the faeces and mucosa was determined with DNA sequencing and BLAST. We showed that the bacterial diversity in the faeces of CD patients was reduced compared with that in healthy controls (p ,0.01). The faecal bacterial dysbiosis of the patients was characterized by an elevated abundance of c-Proteobacteria (especially Escherichia coli and Shigella flexneri) and a reduced proportion of Bacteroidetes and Firmicutes. Five bacterial species defined the microbiota imbalance of the ulcerated mucosa in CD, inclu