human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles人类胚胎干细胞来源于胚胎在发展的不同阶段,分享相似的转录概况.pdfVIP

Human Embryonic Stem Cells Derived from Embryos at Different Stages of Development Share Similar Transcription Profiles 1 1¤ 2 1 Gnanaratnam Giritharan , Dusko Ilic , Matthew Gormley , Ana Krtolica * 1 SLL Sciences, StemLifeLine, Inc., San Carlos, California, United States of America, 2 University of California San Francisco, San Francisco, California, United States of America Abstract We have derived hESC from biopsied blastomeres of cleavage stage embryos under virtually the same conditions we used for the derivation of hESC lines from inner cell mass of blastocyst stage embryos. Blastomere-derived hESC lines exhibited all the standard characteristics of hESC including undifferentiated proliferation, genomic stability, expression of pluripotency markers and the ability to differentiate into the cells of all three germ layers both in vitro and in vivo. To examine whether hESC lines derived from two developmental stages of the embryo differ in gene expression, we have subjected three blastomere-derived hESC lines and two ICM-derived hESC lines grown under identical culture conditions to transcriptome analysis using gene expression arrays. Unlike previously reported comparisons of hESC lines which demonstrated, apart from core hESC-associated pluripotency signature, significant variations in gene expression profiles of different lines, our data show that hESC lines derived and grown under well-controlled defined culture conditions adopt nearly identical gene expression profiles. Moreover, blastomere-derived and ICM-derived hESC exhibited very similar transcriptional profiles independent of the developmental stage of the embryo from which they originated. Furthermore, this profile was evident