human embryonic and fetal mesenchymal stem cells differentiate toward three different cardiac lineages in contrast to their adult counterparts人类胚胎和胎儿间充质干细胞分化向三个不同的心脏血统与成人同行.pdfVIP

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human embryonic and fetal mesenchymal stem cells differentiate toward three different cardiac lineages in contrast to their adult counterparts人类胚胎和胎儿间充质干细胞分化向三个不同的心脏血统与成人同行.pdf

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human embryonic and fetal mesenchymal stem cells differentiate toward three different cardiac lineages in contrast to their adult counterparts人类胚胎和胎儿间充质干细胞分化向三个不同的心脏血统与成人同行

Human Embryonic and Fetal Mesenchymal Stem Cells Differentiate toward Three Different Cardiac Lineages in Contrast to Their Adult Counterparts 1 ¨ 1 ¨ 1 2 1,3 Arti A. Ramkisoensing , Danie l A. Pijnappels , Saıd F. A. Askar , Robert Passier , Jim Swildens , Marie ´ 3, Cindy I. Schutte 1, Antoine A. F. de Vries 1, Sicco Scherjon4, Christine L. Mummery2, Jose Goumans Martin J. Schalij1, Douwe E. Atsma 1* 1 Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands, 2 Department of Embryology and Anatomy, Leiden University Medical Center, Leiden, The Netherlands, 3 Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands, 4 Department of Obstetrics and Gynaecology, Leiden University Medical Center, Leiden, The Netherlands Abstract Mesenchymal stem cells (MSCs) show unexplained differences in differentiation potential. In this study, differentiation of human (h) MSCs derived from embryonic, fetal and adult sources toward cardiomyocytes, endothelial and smooth muscle cells was investigated. Labeled hMSCs derived from embryonic stem cells (hESC-MSCs), fetal umbilical cord, bone marrow, amniotic membrane and adult bone marrow and adipose tissue were co-cultured with neonatal rat cardiomyocytes (nrCMCs) or cardiac fibroblasts (nrCFBs) for 10 days, and also cultured under angiogenic conditions. Cardiomyogenesis was assessed by human-specific immunocytological analysis, whole-cell current-clamp recordings, human-specific qRT-PCR and optical mapping. After co-culture with nrCMCs, significantly more hESC-MSCs than fetal hMSCs stained positive for a-actinin, whereas adult hMSCs stained