molecular typing of treponema pallidum a systematic review and meta-analysis梅毒螺旋体分子类型的系统回顾和荟萃分析.pdfVIP

Molecular Typing of Treponema pallidum : A Systematic Review and Meta-Analysis 1 1,2 1 3 1 1 Rui-Rui Peng , Alberta L. Wang , Jing Li , Joseph D. Tucker , Yue-Ping Yin , Xiang-Sheng Chen * 1 National Center for STD Control, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Dermatology, Nanjing, China, 2 The University of Texas Health Science Center at Houston, Houston, Texas, United States of America, 3 Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts, United States of America Abstract Background: Syphilis is resurgent in many regions of the world. Molecular typing is a robust tool for investigating strain diversity and epidemiology. This study aimed to review original research on molecular typing of Treponema pallidum (T. pallidum) with three objectives: (1) to determine specimen types most suitable for molecular typing; (2) to determine T. pallidum subtype distribution across geographic areas; and (3) to summarize available information on subtypes associated with neurosyphilis and macrolide resistance. Methodology/Principal Findings: Two researchers independently searched five databases from 1998 through 2010, assessed for eligibility and study quality, and extracted data. Search terms included ‘‘Treponema pallidum,’’ or ‘‘syphilis,’’ combined with the subject headings ‘‘molecular,’’ ‘‘subtyping,’’ ‘‘typing,’’ ‘‘genotype,’’ and ‘‘epidemiology.’’ Sixteen eligible studies were included. Publication bias was not statistically significant by the Begg rank correlation test. Medians, inter- quartile ranges, and 95% confidence intervals were determined for DNA extraction and full typing efficiency.