human erythrocytes selectively bind and enrich infectious hiv-1 virions人类红细胞选择性结合,充实感染hiv - 1病毒粒子.pdfVIP

Human Erythrocytes Selectively Bind and Enrich Infectious HIV-1 Virions 1,2 1,2 1,2 1,2 1 Zoltan Beck , Bruce K. Brown , Lindsay Wieczorek , Kristina K. Peachman , Gary R. Matyas , 1 1 1 Victoria R. Polonis , Mangala Rao , Carl R. Alving * 1 Division of Retrovirology, United States Military HIV Research Program, Walter Reed Army Institute of Research, Rockville, Maryland, United States of America, 2 Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland, United States of America Abstract Although CD4(+) cells represent the major target for HIV infection in blood, claims of complement-independent binding of HIV-1 to erythrocytes and the possible role of Duffy blood group antigen, have generated controversy. To examine the question of binding to erythrocytes, HIV-1 was incubated in vitro with erythrocytes from 30 healthy leukapheresis donors, and binding was determined by p24 analysis and adsorption of HIV-1 with reduction of infectivity for CD4(+) target cells. All of the cells, regardless of blood group type, bound HIV-1 p24. A typical preparation of erythrocytes bound ,2.4% of the added p24, but erythrocytes selectively removed essentially all of the viral infectivity as determined by decreased infection of CD4(+) target cells; however, cell-associated HIV-1 was approximately 100-fold more efficient, via trans infection, than unadsorbed virus for infection of CD4(+) cells. All of the bound HIV-1 p24 was released by treatment of the cells with EDTA, and binding was optimized by adding Ca2+ and Mg2+ during the washing of erythrocytes containing bound HIV-1. Although the smal