monitoring bacterial community of human gut microbiota reveals an increase in lactobacillus in obese patients and methanogens in anorexic patients监测细菌的人类肠道微生物群社区揭示乳酸菌在肥胖病人和产甲烷菌的增加厌食症患者.pdfVIP

Monitoring Bacterial Community of Human Gut Microbiota Reveals an Increase in Lactobacillus in Obese Patients and Methanogens in Anorexic Patients 1 1 2 3 1 Fabrice Armougom , Mireille Henry , Bernard Vialettes , Denis Raccah , Didier Raoult * ´ ´ ´ ´ ´ ´ 1 URMITE - UMR CNRS 6236, IRD 3R198, Universite de la Mediterranee, Faculte de medecine, Marseille, France, 2 Service de Nutrition, Maladies Metaboliques et ´ ´ ´ Endocrinologie, UMR Universite mediterranee-INRA U1260, CHU de la Timone, Marseille, France, 3 Nutrition and Diabetology Department, University Hospital Sainte Marguerite, Marseille, France Abstract Background: Studies of the bacterial communities of the gut microbiota have revealed a shift in the ratio of Firmicutes and Bacteroidetes in obese patients. Determining the variations of microbial communities in feces may be beneficial for the identification of specific profiles in patients with abnormal weights. The roles of the archaeon Methanobrevibacter smithii and Lactobacillus species have not been described in these studies. Methods and Findings: We developed an efficient and robust real-time PCR tool that includes a plasmid-based internal control and allows for quantification of the bacterial divisions Bacteroidetes, Firmicutes, and Lactobacillus as well as the methanogen M. smithii. We applied this technique to the feces of 20 obese subjects, 9 patients with anorexia nervosa, and 20 normal-weight healthy controls. Our results confirmed a reduction in the Bacteroidetes community in o