human embryonic stem cell-derived oligodendrocyte progenitors aid in functional recovery of sensory pathways following contusive spinal cord injury人类胚胎干细胞少突细胞祖细胞帮助感觉通路contusive脊髓损伤后的功能恢复.pdfVIP

Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitors Aid in Functional Recovery of Sensory Pathways following Contusive Spinal Cord Injury 1,2 1 1 1 1 1 Angelo H. All *, Faith A. Bazley , Siddharth Gupta , Nikta Pashai , Charles Hu , Amir Pourmorteza , Candace Kerr3* 1 Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 2 Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 3 Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, Maryland, United States of America Abstract Background: Transplantations of human stem cell derivatives have been widely investigated in rodent models for the potential restoration of function of neural pathways after spinal cord injury (SCI). Studies have already demonstrated cells survival following transplantation in SCI. We sought to evaluate survival and potential therapeutic effects of transplanted human embryonic stem (hES) cell-derived oligodendrocyte progenitor cells (OPCs) in a contusive injury in rats. Bioluminescence imaging was utilized to verify survivability of cells up to 4 weeks, and somatosensory evoked potential (SSEPs) were recorded at the cortex to monitor function of sensory pathways throughout the 6-week recovery period. Principal Findings: hES cells were transduced with the firefly luciferase gene and differentiated into OPCs. OPCs were transplanted into the lesion epicenter of rat spinal cords 2 hours after inducing a moderate contusive SCI. The hES- treatment group showed improved SSEPs, including increas