host responses to intestinal microbial antigens in gluten-sensitive mice主机对肠道微生物抗原对小鼠的反应.pdfVIP

Host Responses to Intestinal Microbial Antigens in Gluten-Sensitive Mice 1 1 1 1 2 3 Jane M. Natividad , Xianxi Huang , Emma Slack , Jennifer Jury , Yolanda Sanz , Chella David , 1 4 5 1. ´ 1. Emmanuel Denou , Pinchang Yang , Joseph Murray , Kathy D. McCoy , Elena F. Verdu * 1 Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Canada, 2 Institute of Agrochemistry and Food Technology (IATA), Spanish National Research Council (CSIC), Valencia, Spain, 3 Department of Immunology, Mayo Clinic, Rochester, Minnesota, United States of America, 4 Department of Pathology, McMaster University, Hamilton, Canada, 5 Division of Gastroenterology, Mayo Clinic, Rochester, Minnesota, United States of America Abstract Background and Aims: Excessive uptake of commensal bacterial antigens through a permeable intestinal barrier may influence host responses to specific antigen in a genetically predisposed host. The aim of this study was to investigate whether intestinal barrier dysfunction induced by indomethacin treatment affects the host response to intestinal microbiota in gluten-sensitized HLA-DQ8/HCD4 mice. Methodology/Principal Findings: HLA-DQ8/HCD4 mice were sensitized with gluten, and gavaged with indomethacin plus gluten. Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q-real-time PCR; immune response by in-vitro antigen-specific T- cell proliferation and cytokine analys