heteromeric anopheline odorant receptors exhibit distinct channel propertiesheteromeric按有气味的受体表现出截然不同的信道特性.pdfVIP

Heteromeric Anopheline Odorant Receptors Exhibit Distinct Channel Properties 1 1 ¨ 1,2 3 1,4 Gregory M. Pask , Patrick L. Jones , Michael Rutzler , David C. Rinker , Laurence J. Zwiebel * 1 Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America, 2 The Water and Salt Research Center, Institute of Anatomy, University of Aarhus, Aarhus C, Denmark, 3 Center for Human Genetics Research, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America, 4 Department of Pharmacology, Centers for Molecular Neuroscience and Human Genetics Research, Institutes of Chemical Biology and Global Health and Program in Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America Abstract Background: Insect odorant receptors (ORs) function as odorant-gated ion channels consisting of a conventional, odorant- binding OR and the Orco coreceptor. While Orco can function as a homomeric ion channel, the role(s) of the conventional OR in heteromeric OR complexes has largely focused only on odorant recognition. Results: To investigate other roles of odorant-binding ORs, we have employed patch clamp electrophysiology to investigate the properties of the channel pore of several OR complexes formed by a range of different odorant-specific Anopheles gambiae ORs (AgOrs) each paired with AgOrco. These studies reveal significant differences in cation permeability and ruthenium red susceptibility among different AgOr complexes. Conclusions: With observable differences in channel function, the data support a model in which the odorant-binding OR also affects the channel pore. The v