heterosubtype neutralizing responses to influenza a (h5n1) viruses are mediated by antibodies to virus haemagglutininheterosubtype中和应对甲型流感病毒(h5n1)由病毒血凝素抗体介导的.pdfVIP
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heterosubtype neutralizing responses to influenza a (h5n1) viruses are mediated by antibodies to virus haemagglutininheterosubtype中和应对甲型流感病毒(h5n1)由病毒血凝素抗体介导的
Heterosubtype Neutralizing Responses to Influenza A (H5N1) Viruses Are Mediated by Antibodies to Virus Haemagglutinin 1 2 ` 1 3 2 Jean-Michel Garcia *, Stephanie Pepin , Nadege Lagarde , Edward S. K. Ma , Frederick R. Vogel , 3 4 1,3 Kwok H. Chan , Susan S. S. Chiu , J. S. M. Peiris * 1 HKU-Pasteur Research Centre, Hong Kong Special Administrative Region, China, 2 Sanofi Pasteur, Marcy l’Etoile, France, 3 Department of Microbiology, University of Hong Kong, Hong Kong Special Administrative Region, China, 4 Department of Paediatrics and Adolescent Medicine, University of Hong Kong, Hong Kong Special Administrative Region, China Abstract Background: It is increasingly clear that influenza A infection induces cross-subtype neutralizing antibodies that may potentially confer protection against zoonotic infections. It is unclear whether this is mediated by antibodies to the neuraminidase (NA) or haemagglutinin (HA). We use pseudoviral particles (H5pp) coated with H5 haemagglutinin but not N1 neuraminidase to address this question. In this study, we investigate whether cross-neutralizing antibodies in persons unexposed to H5N1 is reactive to the H5 haemagglutinin. Methodology/Principal Findings: We measured H5-neutralization antibody titers pre- and post-vaccination using the H5N1 micro-neutralization test (MN) and H5pp tests in subjects given seasonal vaccines and in selected sera from European elderly volunteers in a H5N1 vaccine trial who had detectable pre-vaccination H5N1 MN antibody titers. We found detectable (titer $20) H5N1 neutralizing antibo
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