中药复方及单味药材中淫羊藿苷大鼠体内药动学分析.docVIP

中药复方及单味药材中淫羊藿苷大鼠体内药动学分析[摘要] 目的:利用反相高效液相色谱法,分别比较复方与单味药材提取物中淫羊藿苷在大鼠体内的药代动力学(药动学)参数差别,探讨配伍对药效成分体内过程的影响。方法:实验大鼠分别灌胃复方及单味药材提取物后,测定不同时间点血浆中淫羊藿苷的浓度,采用药动学程序3P97对数据进行统计学处理,进行药动学模型拟合并计算相关药代动力学参数。结果:复方及单味药材中淫羊藿苷在大鼠体内分别呈二室和一室模型,主要药代动力学参数为:复方组Cmax=(3.133±0.290) μg/ml,t1/2(Ka)=(0.753±0.809) h,Tmax=(2.089±0.144) h,AUC0-24=(30.839±3.680) μg?h/ml。单味药材组Cmax=(2.318±0.218) μg/ml,Tmax=(1.497±0.521) h,AUC0-24=(19.947±2.555) μgh/ml。结论:与单味药材相比,复方中淫羊藿苷在大鼠体内吸收增加、消除及达峰时间延长、血浆清除率减少,因此复方配伍对淫羊藿苷体内过程有显著影响。 [关键词] 药代动力学;淫羊藿;淫羊藿苷;HPLC [中图分类号]R965 [文献标识码] A[文章编号] 1673-7210(2009)07(a)-081-04 Pharmacokinetics analysis of icariin in rats coming from Chinese medicine compound recipe and single medicinal materials DUAN Xuguang, ZHOU Chongwen, CHEN Ke, ZENG Lei (Hunan Environment and Biologic Polytechnic, Hengyang 421005, China) [Abstract] Objective: To compare the difference of kinetic parameter of medication-metabolism in rats of the icarrin, which come from compound recipe and single medicinal materials using RP-HPLC, and disscuss the influence of compatibility of medicines to body. Methods: The rats were intragastric administered with compound recipe and extractive of Longspur Epimedium respectively. The blood concentration of different time points were tested by proposed established RP-HPLC. Data were dealed with pharmacokinetic programme, and fitted to kinetic parameter. It showed that icarrin in the compound recipe was in two-chamber in mouse, and single medicinal materials in one-chamber. Results: Main kinetic parameters were that compound group was Cmax=(3.133±0.290) μg/ml, t1/2(Ka)=(0.753±0.809) h, Tmax=(2.089±0.144) h, AUC0-24=(30.839±3.680) μg?h/ml. Single medicinal materials group was Cmax=(2.318±0.218) μg/ml, Tmax=(1.497±0.521) h, AUC0-24=(19.947±2.555) μg?h/ml. Conclusion: The results show that there are some significance differences of icariin between the compound recipe and single herber. The elimination, absorption, peak time of icariin in the compound recipe are higer t