非洲菊切花衰老过程中细胞壁组分及相关酶活性变化.pdfVIP

●蕊 |J：皇 摘 要：研究了非洲菊切花易弯茎品种Sondance衰老过程中细胞壁组分及其相关水解酶活性的 变化。结果表明，花茎纤维素、原果胶含量在瓶插前期均有所增加，3天后迅速下降；瓶插过程 中，可溶性果胶含量与原果胶含量变化呈显著负相关；纤维素酶、多聚半乳糖醛酸酶、果胶甲酯酶 活性在瓶插 1～5天均呈快速上升之后下降。细胞壁物质的分解以及细胞壁水解酶活性的快速上 升是非洲菊切花弯茎的主要原因。 关键词：非洲菊；切花；细胞壁组分；细胞壁水解酶；衰老 Changes of Cell WaⅡComponents and Cell WaⅡ一degrading Enzyme ZHANG Jie，ZHONG Feng-lin，GUO Zhi-xiong，PAN Dong—ming (Department of Horticulture，Fujian Agriculture and Forestry University，Fuzhou 350002，China) Abstract：The changes in cell wall component and cell wall—·degrading enzyme were investigated during senescence ofGerberajamesonii Bolus．cv．Sondance cut-flower．The results showed that the cellulose，protopectin content ofthe scapes increased at the early stage of vase holding，and decreased rapidly after 3 days．The changes in soluble pectin content and protopectin content showed obviously negative correlation．Cellulase，pectinesterase and polygalactur- onase activity increased rapidly from 1 d to 5 d during vase holding,and then decreased．It was concluded that the decomposition of cell wall component and rising of cell wall-degrading enzyme activity were considered to be the main causes of bend scape of Gerbera jamesonii cut-flower． Key words：Gerberajamesonii；cut-flower；cell wall component；cell wall—·degrading enzyme；senescence 中图分类号：$682．1+9 文献标识码：A 文章编号：1009—6221(2008)02—0031—03 非洲菊(Gerbera amesonii Bolus)又名扶郎花， 水分吸收有关，Mencarelli(1995)$Jk为与乙烯调 属菊科大丁草属多年生常绿宿根草本花卉。花朵 有关，廖立新等(2003)则认为与弯茎部位的粗 硕大，花枝挺拔，花色丰富，被称为第五大切花。非 维含量有关。目前，关于非洲菊切花弯茎机理还 洲菊采后贮运和瓶插过程中易出现花头下垂、花 有比较一致的看法。植物细胞壁组分是细胞壁机 茎弯曲现象(常称弯茎)而降低观赏价值。对非洲 械性能的物质基础，也是花茎强度的重要物质基 菊切花弯茎机理的研究，黄绵佳等(2003)认为与 础。本试验研究了非洲菊切花衰老过程中，细胞壁 组分及细胞壁水解酶活性的变化，旨在从细胞壁 机械强度方面探讨非洲菊切花弯茎机理，寻找延 缓非洲菊