高效液相色谱法检测水产品中磺胺类和乙胺嘧啶药物残留.pdfVIP

--_J Cj 同 效液相色谱法检测水产品中磺胺类 和乙胺嘧啶药物残留 李佐卿，倪梅林，章再婷，谢东华，俞雪钧，湛 嘉，殷居易 (宁波出入境检验检疫局，宁波315012) 摘 要：用高效液相色谱法检测水产品中磺胺类、乙胺嘧啶等合成抗茵剂残留。样品以乙腈、二 氯甲烷、乙酸乙酯以体积比2比l比l为提取剂，经脱脂、净化、浓缩，用流动相溶解。用高效液相 色谱法紫外检测器测定。通过梯度洗脱将7种磺胺类、乙胺嘧啶进行分离。各标准曲线线性范围 为0。O5～2。0 mg·I ～，相关系数为0．983 7～O。997 5，回收率分别为62．2％～88．6 ，相对标准 偏差为5。85 ～8。12 (72—7)，检出限(s／N一3)为10~50 fig·kg～ 。 关键词：高效液相色谱；磺胺类；乙胺嘧啶 中图分类号：0657．7 文献标识码：A 文章编号：1001—4020(2008)06—0557-03 HPLC Determination of Residual Sulfanilamides and Ethylaminopyrimidines in Aquatic Products LI Zuo-qing，NI Mei—lin，ZHANG Zai—ting，XIE Dong-hua，YU Xue-jan，ZHAN Jia，YIN Ju-yi (Ningbo Entry-Exit Inspection and Quarantine Bureau，Ningbo 315012，China) Abstract：HPLC was applied to the determination of residual sulfanilamides and ethylaminopyrimidines in aquatic products。The residua1 drugs in the sample were extracted with a solvent mixture of acetonitrile， dichloromethan and ethyl acetate mixed in the proportion of 2；1：1 respectively．The extract obtained was defatted and purified by extraction with hexane，and the hexane extract was then concentrated by evaporation．The residue was taken up with a mixed solvent of acetonitrile，acetic acid(2+98)and H2O mixed in the ratio of 154-254-6O， which was used also as the mobile phase in the chromatographic separation later．Seven kinds of sulfanilamide and ethylaminopyrimidine drugs were well separated by gradient elution On the chromatographic column of Clovisl-C18 (4．6 mmX 150 mill，5 m)，and their contents determ ined by UV-detection at the wavelength of 275 nlTL Linear relationship between the peak area and the concentration of standards of the 7 different drugs were found all in range of 0．O5—2．0 mg·L ，with correlation coefficients