高效液相色谱法同时测定山豆根中苦参碱和氧化苦参碱含量.pdfVIP

2008年第17卷第14期 药物鉴定 高效液相色谱法同时测定山豆根中苦参碱和氧化苦参碱含量 袁祥慧，李绪伦 (重庆市药品检验所，重庆 401121) 摘要：目的 建立同时测定山豆根中苦参碱和氧化苦参碱含量的方法。方法 用三氯甲烷超声提取，通过中性氧化铝柱，依次以三氯甲烷、三 氯甲烷一甲醇洗脱。色谱柱以氨基键舍硅胶为填充剂(250 mm×4．6 mm，5 Ixm)，流动相为乙腈一无水乙醇一3％磷酸溶液(8O：1O：1O)，流 速为1．0 mL／min，检测波长205 nm，柱温30~C。结果 苦参碱进样量在0．102 1—4．082 Ixg范围内与峰面积值呈良好的线性关系(r= 0．999 9)，氧化苦参碱进样量在0．194 3—3．886 Ixg范围内与峰面积值呈良好的线性关系(r=0．999 8)，平均回收率均在99％以上。 结论 方法准确、灵敏度高、重现性好，能同时测定山豆根中苦参碱和氧化苦参碱的含量，可作为山豆根药材质量控制的含量检测方法。 关键词：山豆根；苦参碱；氧化苦参碱；高效液相色谱法；含量测定 中图分类号：11284．1；11282．71 文献标识码：A 文章编号：1006—4931(2008)14—0031—02 Simultaneous Determination of Matrine and Oxymatrine in Sophora Tonkinensis Gapnep．by HPLC Yuan Xianghui，Li Xulun f Chongqing Jm u For Drug Control，Chongqing，China 401 121 J Abstract：Objective To developed a method for determining matrine and oxymatrine simultaneously in the dried root of Sophora tonkinensis Gapnep．by HPLC．Methods The YWG—NH2 column(250 mm×4．6 mm，5 Ixm)with the solvent system consisting of acetonitrile—anhydrous ethanol一3％ phosphoric acid solution(80：10：10)was used．The detection wavelength was at 205 nm by UV spectrometry and the column temperature was 30oC．The flow rate was 1．0 mL／min．Results During 0．1 02 1—4．082 Ixg。the linear regression of matrine was favorable， and during 0．194 3—3．886 Ixg， the linear regression of oxymatrine was favorable．The average recovery rates were all more than 99％． Conclusion The method is simple， rapid and sensitive．It can be used for the simultaneous determination of matrine and oxymatrine in Sophora tonkinensis Gapnep． Key words：Sophora tonkinensis Gapnep；matrine；oxymatrine；HPLC；content determination 山豆根为豆科植物越南槐Sophora tonkinens~Gapnep．的干燥 图1。 根及根茎，具有清热解毒、消肿利咽的功效，用于火毒蕴结、咽喉肿