胃转流术对GK大鼠炎症反应的影响.pdfVIP

减少，这可能是胃转流术治疗糖尿病的机制之一。 关键词：胃转流术；2 型糖尿病；GK 大鼠；胰岛炎症；胰岛素抵抗； 纤维化；白介素-1 β 5 Effects of gastric bypass surgery on the inflammatory of GK rats Abstract: Roux-en-Y gastric bypass surgery is a new method of curing type-2 diabetes with a high efficiency. But, up to now, its mechanism is not totally clear to us. Insulitis and inflammatory in blood circulation are the important factors of the development of type-2 diabetes. Inflammatory in blood circulation such as IL-1β, TNF-a will aggravate insulin resistance of tissues; and the insulitis will aggravate beta-cell damage and mediate beta-cell apoptosis, and finally lead to the fibration of islets of langerhans and to the happening and aggravation of diabetes. Objective: The purpose of the present experiment is to observe gastric bypass surgery’s effect on the inflammatory reaction of GK rat, discuss the potential mechanism to curing diabetes of gastric bypass surgery. Methods: GK rat was used as experimental diabetic animal model, SPF-class healthy male GK rats were randomly divided into two groups: 20 for operation group and 20 for sham operation group. Carry out gastric bypass surgery to the operation group, and transection and reanastomosis of the jejunum tract to the sham operation group. Measure the weight and fasting glucose of the two groups of rats respectively before operation and 1, 2and 4 weeks after operation, and do the OGTT experiment to them. Before and 4 weeks after operation, detect insulin expression in serum of each groups’ rats by radioactive immunoassay, calculate the numbers of IRI and IAI. Before operation and 4 6 weeks after operation detect