胃镜取样的良性和恶变组织的分子标志筛选.pdfVIP

IDENTIFICATION OF THE MOLECULAR MARKERS TO DISTINGUISH MALIGNANT NEOPLASIA OF GASTROSCOPICALLY OBTAINED GASTRIC MUCOSA FROM BENIGN ONES ABSTRACT Gastric cancer is a common cancer, the mortality rate remain second in the world. incidence of gastric cancer is high in China with a still high mortality. The 5-year survival rate of gastric cancer depend on its depth of invasion, so early diagnosis of gastric cancer and a radical treatment is the only way to a good prognosis. Gastroscopy combined with pathological examination greatly improved the early diagnosis of gastric cancer, but the misdiagnosis rate of gastric cancer before surgery is still up 10% to 20% because of the restriction of physicians technical proficiency and pathological doctors judgement. The development of new molecules diagnosis system for the prevention and treatment of gastric cancer is essential. So this search is designed to identify molecular markers by comparing expression profile between pathologic diagnosed malignant and benign gastric mucosa changes. gastric mucosa samples with malignant and benign pathological changes, along with contra-lateral gastric mucosa were gastroscopically obtained in 35 patients, RNA was extracted, amplified and hybridized to Oligo microarray containing 1299 spots prepared by our laboratory. The data obtained were normalized with locally weighted linear regression (LOWESS) Method. The significance of intragroup difference between pathological changes and contra-lateral samples is analyzed by significance analysis of microarray (SAM) and the intergroup difference between malignant and benign pathological changed samples was analyzed by t-TEST（P 0.01）.Real-time PCR was applied as a verification method. 367 genes ar