uv-a诱导芜花青素合成相关基因表达特性研究及全长克隆.pdf

摘 费 摘 要 个K克隆。 卜要研究结果如下： UV．A诱导。 |忖削的延长基因的表达量逐渐增加。 化。 1 1 70bp，包含一个完整的』f放阅读框(openreading 卜游有一段长9bp的5’非编码区，在终止密码子下游还包含长84bp的3’非编码区．表 }WRACE反应得至0的cDNA全长序列比较完整。 关键词花青素；UV—A；芜菁：丹Ⅳ Abstract Twoeultivarsof in of turnip(Brassica campestrisL．ssp．rapa)differentlight in ofathickrootwere synthesis anthocyanin peel light· insensitive。Yurugi—Akamaru’turnip． Root above levelin parts ground bothcultivarswere inthedarkor exposed kept with RNAwas UV·A．Total extractedfromroot wasconducted peels．Northemblotting using lbur of constitutive and probesanthocyaninsynthesismajor ANS, genes，i．e．，PAL．F3以DFR a ofaMYB·related factoranda UV-A CRY2 probe transcriptiongene of probe tOdetectthe charactersofthese UV·A gene expression Theeffectof toa genes thickroot on characterswas in‘Tsuda’turnipexpression analyzed．A ofeDNA full of of wasconductedtoobtainthe eDNAa amplificationends)analysis length F3H． keygene resultsaresummarizedasfollows． Major (1)PAL．F3麒DF