消癌平注射液下调MMP9基因表达抑制卵巢癌细胞Caov3迁移机制地研究.pdfVIP

886 中国肿瘤临床 2012年第39卷第13期 消癌平注射液下调MMP-9基因表达抑制卵巢癌细 胞Caov-3迁移机制的研究* 王 淳 董 秀 王 梅 王晓波 研究中药消癌平注射液下调MMP-9基因表达，抑制卵巢癌细胞Caov-3迁移的机制。 利用消癌平注射液 摘要 目的： 方法： 干预体外培养的卵巢癌Caov-3细胞，分别应用：细胞划痕实验观察细胞的迁移水平；肿瘤细胞侵袭实验（Transwell小室法）观察细 胞侵袭能力；RT-PCR实验检测卵巢癌细胞中金属蛋白酶MMP-9基因表达水平。以金属蛋白酶MMPs特异性抑制剂-Doxycyclin 作为阳性对照，同时应用PBS作空白对照。 消癌平可有效抑制Caov-3细胞迁移，与空白对照组相比差异有统计学意义（P 结果： 0.05），但与Doxycyclin组差异无统计学意义（P0.05）；消癌平还可部分抑制血清诱导的Caov-3细胞穿过Transwell小室，其抑制效 果与Doxycyclin相似；此外，消癌平还能够下调MMP-9基因表达水平。 消癌平下调MMP-9基因表达，抑制卵巢癌Caov-3细 结论： 胞迁移。 关键词 消癌平 卵巢癌 MMP-9 细胞迁移 doi:10.3969/j.issn.1000-8179.2012.13.003 Xiaoaiping Injection Inhibits Cell Migration by Reducing MMP-9 Gene Expression in Human Ovarian Cancer Cells Chun WANG, Xiu DONG, Mei WANG, Xiaobo WANG Correspondence to: Chun WANG; E-mail: chunwang2000@ Department of Hygiene and Biology, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China This work was supported by the Natural Science Fund of Liaoning Province (No. 2008441) and the Talents Fund of Liaoning University of Traditional Chinese Medicine Abstract Objective: To study the inhibitory mechanism of an injection of the Chinese medicine Xiaoaiping ( XAP ) on the migration of human ovarian cancer Caov-3 cells via reduced matrix metalloproteinase-9 ( MMP-9 ) gene expression Methods: The ovarian cancer Caov-3 cells were treated with XAP in vitro. The inhibitor doxycyclin was also applied to the MMPs as the positive control, whereas phosphate-buffered saline served as the blank control. Wound healing and cell invasion assays using Transwell chambe